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使用环保型溶剂在常压和高压下从杜松叶中回收抗癌鬼臼毒素

Anticancer Podophyllotoxin Recovery from Juniper Leaves at Atmospheric and High Pressure Using Eco-Friendly Solvents.

作者信息

Ivanova Diana, Nedialkov Paraskev, Tashev Alexander, Kokanova-Nedialkova Zlatina, Olech Marta, Nowak Renata, Boyadzhieva Stanislava, Angelov George, Yankov Dragomir

机构信息

Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.

Department of Pharmacognosy, Faculty of Pharmacy, Medical University, 1000 Sofia, Bulgaria.

出版信息

Plants (Basel). 2023 Mar 31;12(7):1526. doi: 10.3390/plants12071526.

DOI:10.3390/plants12071526
PMID:37050153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10096874/
Abstract

Podophyllotoxin (PPT) is a precursor for the synthesis of drugs against cancer and other diseases. The present sources of PPT ( and ) are endangered species, with PPT production highly dependent on their growing conditions. In connection with the identification of new sources of PPT, the present study aimed to recover PPT from leaves via atmospheric or high pressure extraction methods with a focus on using eco-friendly solvents. PPT quantification was determined by UHPLC/HRMS/MS. A thorough study of conventional extraction was carried out to reveal the optimal conditions (solvent ethyl acetate at room temperature and a duration of 1 h) for maximizing the PPT recovery (about 30 mg/g of dry extract and 3 mg/g of dry initial plant material). Peleg's equation was applied for process kinetics modeling. The best PPT content in the final dry extract (42-45 mg/g of dry extract) was obtained by high pressure methods under supercritical (scCO with ethanol or ethyl acetate, 30 MPa, 50 °C and 100 min) or accelerated solvent extraction conditions (solvent ethyl acetate, 10.35 MPa, 20 °C and 3 cycles for 15 min). Seasonal stability and storage stability of the raw material were also determined. The present results have potential applications in the pharmacy for the delivery of PPT from juniper leaves.

摘要

鬼臼毒素（PPT）是合成抗癌及其他疾病药物的前体。目前PPT的来源（及）均为濒危物种，PPT的生产高度依赖其生长条件。鉴于需要确定PPT的新来源，本研究旨在通过常压或高压萃取方法从杜松叶中回收PPT，重点是使用环保型溶剂。通过超高效液相色谱/高分辨质谱/质谱法测定PPT含量。对传统萃取进行了深入研究，以揭示使PPT回收率最大化的最佳条件（室温下使用乙酸乙酯溶剂，持续1小时）（约30毫克/克干提取物和3毫克/克干初始植物材料）。应用佩莱格方程进行过程动力学建模。通过超临界（scCO₂与乙醇或乙酸乙酯，30兆帕，50℃和100分钟）或加速溶剂萃取条件（溶剂乙酸乙酯，10.35兆帕，20℃和3个15分钟循环）下的高压方法，在最终干提取物中获得了最佳的PPT含量（42 - 45毫克/克干提取物）。还测定了原料的季节稳定性和储存稳定性。本研究结果在制药领域从杜松叶中提取PPT方面具有潜在应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/e50ba41222e9/plants-12-01526-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/b08f3262e313/plants-12-01526-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/3f4ddbd82967/plants-12-01526-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/ed509b8919d8/plants-12-01526-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/082d0b3335f0/plants-12-01526-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/84e2caff4221/plants-12-01526-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/ea5a0fc9327a/plants-12-01526-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/cb30dde714bd/plants-12-01526-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/e50ba41222e9/plants-12-01526-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/b08f3262e313/plants-12-01526-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/3f4ddbd82967/plants-12-01526-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/ed509b8919d8/plants-12-01526-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/082d0b3335f0/plants-12-01526-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/84e2caff4221/plants-12-01526-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/ea5a0fc9327a/plants-12-01526-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/cb30dde714bd/plants-12-01526-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2c0/10096874/e50ba41222e9/plants-12-01526-g008.jpg

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