Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
J Ocul Pharmacol Ther. 2011 Aug;27(4):323-6. doi: 10.1089/jop.2011.0007. Epub 2011 Jun 24.
The aim of the study was the quantitative pharmacokinetic evaluation of drug release from pretreated amniotic membrane (AM) in vitro.
Cryopreserved AM pieces soaked in 3% ofloxacin ophthalmic solution were mounted in vertical Franz-diffusion cell system equipped with autosampler. In vitro release of ofloxacin was determined by quantitative absorbance measurement carried out with a UV spectrophotometer (wavelength 287 nm). Three groups were created according to the duration of soaking: 60 (Group 1), 120 (Group 2), and 180 (Group 3) minutes. Released amount of ofloxacin pro 1 cm(2) of AM (μg/cm(2)) was calculated in the period of 1 to 450 min.
Ofloxacin was detectable in the acceptor phase 1 min after mounting in all groups. Until 120 min, rapid increase of released ofloxacin could be observed. From 120 to 450 min, the amount of released ofloxacin showed a slower increasing pattern. Released ofloxacin in Group 1 was significantly lower than in Group 2 after 90 min (19.4±10.4 μg/cm(2), 51.6±20.7 μg/cm(2), respectively, P=0.044). In Group 3, cumulative drug release was higher than in Group at all timepoints. No significant difference could be demonstrated between Groups 2 and 3 at only 1 min timepoint.
Significant ofloxacin reservoir capacity of a single human amniotic layer could be demonstrated in vitro. AM acted as an ofloxacin slow release device for upto 7 h in vitro, depending on the duration of pretreatment of AM. Individual pretreatment of AM could increase beneficial effects of AM transplantation, especially in infectious keratitis.
本研究旨在对预处理羊膜（AM）体外药物释放的定量药代动力学进行评估。
将浸泡在 3%左氧氟沙星滴眼液中的冷冻 AM 片安装在配备自动进样器的垂直 Franz 扩散细胞系统中。通过使用紫外分光光度计（波长 287nm）进行定量吸光度测量来确定左氧氟沙星的体外释放。根据浸泡时间将样本分为三组：60 分钟（组 1）、120 分钟（组 2）和 180 分钟（组 3）。在 1 至 450 分钟的时间段内计算 AM 每 1cm²释放的左氧氟沙星量（μg/cm²）。
所有组在安装后 1 分钟时即可在接受液相中检测到左氧氟沙星。在 120 分钟之前，可以观察到释放的左氧氟沙星迅速增加。从 120 分钟到 450 分钟，释放的左氧氟沙星呈缓慢增加的模式。在 90 分钟后，组 1 释放的左氧氟沙星明显低于组 2（19.4±10.4μg/cm²，51.6±20.7μg/cm²，P=0.044）。在组 3 中，累积药物释放在所有时间点均高于组 2。仅在 1 分钟时间点，组 2 和组 3 之间未显示出显著差异。
体外研究表明，单层人羊膜具有显著的左氧氟沙星储库能力。AM 作为左氧氟沙星的缓释装置，在体外可维持长达 7 小时的时间，具体时间取决于 AM 预处理的持续时间。AM 的个体化预处理可以增加 AM 移植的有益效果，特别是在感染性角膜炎中。
