Department of Cornea and Refractive Services, Aravind Eye Hospital, Madurai, Tamil Nadu, India.
Department of Ocular Pharmacology, Aravind Medical Research Foundation, Madurai, Tamil Nadu, India.
Indian J Ophthalmol. 2021 May;69(5):1068-1072. doi: 10.4103/ijo.IJO_2649_20.
Earlier our group has demonstrated the drug reservoir function of the human amniotic membrane (HAM) using stable moxifloxacin and fortified cefazolin ophthalmic formulations and found it as a suitable tool to deliver drugs for an extended duration. The purpose of this study was to evaluate the extended-release kinetics of voriconazole from the impregnated human amniotic membrane (HAM) in vitro.
HAM buttons were incubated with freshly prepared 1% topical ophthalmic formulation of voriconazole for 5 different exposure time to investigate the ideal exposure time for the extended-release of voriconazole from HAM. The drug release kinetics was studied in simulated tear fluid for 5 weeks and the amount of voriconazole released at different intervals was estimated using high-performance liquid chromatography (HPLC) with photodiode array (PDA) detector.
There was a marginal increase in drug entrapment efficiency with increased drug exposure time but neither the drug entrapment nor the drug release was found to be statistically significant (P ≥ 0.5). Voriconazole was detectable even at 5 weeks.
A sustained release of voriconazole was achieved up to 5 weeks, when voriconazole was incubated with amniotic membrane for all the studied drug soaking times. Thus, voriconazole impregnated amniotic membrane can be considered for the sustained delivery for its in fungal keratitis.
我们的研究小组先前已经证实，使用稳定的莫西沙星和强化头孢唑林眼科制剂的人羊膜（HAM）具有药物储库功能，并发现其是一种可用于延长药物作用时间的合适工具。本研究旨在评估载唑康唑的人羊膜（HAM）在体外的缓释动力学。
将 HAM 纽扣用新制备的 1%局部眼用唑康唑制剂孵育 5 种不同的暴露时间，以研究从 HAM 中延长释放唑康唑的理想暴露时间。在模拟泪液中研究药物释放动力学 5 周，并使用高效液相色谱法（HPLC）和光电二极管阵列（PDA）检测器在不同时间间隔估计释放的唑康唑量。
随着药物暴露时间的增加，药物包封效率略有增加，但药物包封和药物释放均无统计学意义（P≥0.5）。即使在 5 周时也能检测到唑康唑。
当唑康唑与人羊膜孵育所有研究的药物浸泡时间时，可实现长达 5 周的持续释放。因此，载唑康唑的人羊膜可用于真菌性角膜炎的持续释放。
