McKillop J M, McCann J P, Gibbons J R, Johnston C F, Buchanan K D
Department of Medicine, Queen's University of Belfast, Northern Ireland, UK.
Int J Cancer. 1990 Oct 15;46(4):591-61. doi: 10.1002/ijc.2910460407.
Levels of gastrin-releasing peptide (GRP) were determined by radioimmunoassay in human normal main and lobar bronchus and parenchymal lung tissue extracts. It was found that the level of GRP differed significantly between all 3 areas. The concentration of GRP was statistically higher in main bronchus (median 6.74 ng/g) compared to both lobar bronchus (median 4.79 ng/g) and parenchymal lung (median 1.73 ng/g), and also statistically higher in lobar bronchus compared to parenchymal lung. Chromatographically, GRP-immunoreactivity in both main and lobar bronchial extracts corresponded to GRP1-27 and GRP18-27, while in lung tissue only one major species was identified which corresponded in retention time to GRP18-27. No significant difference was detected when the levels of GRP in normal lobar bronchus and normal lung tissue were compared to the levels in lobar bronchus and lung taken from patients with lung carcinoma, at a site adjacent to the carcinoma. However, a significant difference was observed between the GRP content of normal main bronchus compared to main bronchus from patients with carcinoma. GRP was measured in 26/56 lung carcinomas examined. The levels ranged from 42,000 ng/g in a carcinoid tumour to 0.18 ng/g in a squamous-cell carcinoma, though only in 6 tumours were the levels outside the range determined for normal pulmonary tissue. Chromatography of selected tumour extracts of different histopathologies showed that there were differences in the GRP products present.
采用放射免疫分析法测定人正常主支气管、叶支气管及肺实质组织提取物中胃泌素释放肽（GRP）的水平。结果发现，这三个区域的GRP水平存在显著差异。主支气管中GRP的浓度（中位数为6.74 ng/g）在统计学上高于叶支气管（中位数为4.79 ng/g）和肺实质（中位数为1.73 ng/g），叶支气管中的GRP浓度在统计学上也高于肺实质。色谱分析显示，主支气管和叶支气管提取物中的GRP免疫反应性与GRP1-27和GRP18-27相对应，而在肺组织中仅鉴定出一种主要成分，其保留时间与GRP18-27相对应。将正常叶支气管和正常肺组织中的GRP水平与肺癌患者癌旁叶支气管和肺组织中的GRP水平进行比较时，未检测到显著差异。然而，正常主支气管与癌患者主支气管的GRP含量之间存在显著差异。在所检查的56例肺癌中，对26例进行了GRP检测。其水平范围从类癌肿瘤中的42,000 ng/g到鳞状细胞癌中的0.18 ng/g，不过只有6个肿瘤的水平超出了正常肺组织所确定的范围。对不同组织病理学类型的选定肿瘤提取物进行色谱分析表明，所存在的GRP产物存在差异。
