IncW 质粒 R388 接合转移基因的总体组织以及 R388 与 IncN 和 IncP 质粒之间的相互作用。
General organization of the conjugal transfer genes of the IncW plasmid R388 and interactions between R388 and IncN and IncP plasmids.
作者信息
Bolland S, Llosa M, Avila P, de la Cruz F
机构信息
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
出版信息
J Bacteriol. 1990 Oct;172(10):5795-802. doi: 10.1128/jb.172.10.5795-5802.1990.
Abstract
The complete conjugal transfer gene region of the IncW plasmid R388 has been cloned in multicopy vector plasmids and mapped to a contiguous 14.9-kilobase segment by insertion mutagenesis. The fertility of the cloned region could still be inhibited by a coresident IncP plasmid. The transfer region has been dissected into two regions, one involved in pilus synthesis and assembly (PILW), and the other involved in conjugal DNA metabolism (MOBW). They have been separately cloned. PILW also contains the genes involved in entry exclusion. MOBW contains oriT and the gene products required for efficient mobilization by PILW. MOBW plasmids could also be mobilized efficiently by PILN, the specific pilus of the IncN plasmid pCU1, but not by PILP, the specific pilus of the IncP plasmid RP1.
摘要
IncW 质粒 R388 的完整接合转移基因区域已被克隆到多拷贝载体质粒中，并通过插入诱变定位到一个连续的 14.9 千碱基片段上。克隆区域的育性仍可被共居的 IncP 质粒抑制。转移区域已被分为两个区域，一个参与菌毛的合成与组装（PILW），另一个参与接合 DNA 代谢（MOBW）。它们已被分别克隆。PILW 还包含参与进入排斥的基因。MOBW 包含 oriT 和 PILW 高效动员所需的基因产物。MOBW 质粒也可被 IncN 质粒 pCU1 的特异性菌毛 PILN 高效动员，但不能被 IncP 质粒 RP1 的特异性菌毛 PILP 动员。
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