Cabezón E, Lanka E, de la Cruz F
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
J Bacteriol. 1994 Jul;176(14):4455-8. doi: 10.1128/jb.176.14.4455-4458.1994.
Mobilization of plasmid RSF1010 by the IncW plasmid R388 requires the genes involved in W pilus synthesis plus trwB. traG of the IncP plasmid RP4 can substitute for trwB in RSF1010 mobilization by R388 but not in self-transfer of R388. This result suggests a dual specificity of TrwB-like proteins in conjugation. The same genetic requirements were found for R388 to mobilize the unrelated plasmid ColE1.
IncW质粒R388对质粒RSF1010的动员需要参与W菌毛合成的基因以及trwB。IncP质粒RP4的traG可在R388对RSF1010的动员中替代trwB,但不能在R388的自我转移中替代trwB。这一结果表明TrwB样蛋白在接合过程中具有双重特异性。R388动员不相关质粒ColE1时也发现了相同的遗传要求。