Laboratoire Adaptation et Pathogénie des Micro-organismes, Université Joseph Fourier Grenoble, CNRS UMR 5163, Grenoble, France.
Cell Immunol. 2011;270(2):230-6. doi: 10.1016/j.cellimm.2011.05.013. Epub 2011 May 27.
Phagocytes play a central role in immune defense. Their dysfunction predisposes to infections. This study determined the expression level of nine receptors involved in Aspergillus immune response as well as the values of phagocytosis and production of radical oxygen species after Aspergillus stimulation, in a healthy adult population. The expression values of the CD11b, CD11c, CD14, CD18, CD35, CD181, CD182, CD282 and CD284 receptors on peripheral human monocytes and granulocytes was established. A heterogenous expression of the CD282 on granulocytes was observed as CD181, CD182 and CD284 on monocytes. Similarly, we observed considerable variation in the expression of these receptors over time. Only CD282 on granulocytes varied with sex. No variation with age was observed. Adherence of Aspergillus conidia to phagocytes was dependent of individual, sex, age and time. A better characterization of these innate immunity parameters is necessary to develop in the future an immunologic surveillance strategy for transplant recipients.
吞噬细胞在免疫防御中发挥核心作用。其功能障碍易导致感染。本研究旨在确定健康成年人中参与曲霉菌免疫反应的九个受体的表达水平，以及曲霉菌刺激后吞噬作用和活性氧产生的水平。建立了外周血人单核细胞和粒细胞上的 CD11b、CD11c、CD14、CD18、CD35、CD181、CD182、CD282 和 CD284 受体的表达值。我们观察到粒细胞上的 CD282 呈异质性表达，而单核细胞上的 CD181、CD182 和 CD284 则呈均一性表达。同样，我们观察到这些受体的表达随时间有很大的变化。只有粒细胞上的 CD282 随性别而变化，而与年龄无关。曲霉菌分生孢子与吞噬细胞的黏附依赖于个体、性别、年龄和时间。为了在未来为移植受者开发免疫监测策略，有必要更好地描述这些先天免疫参数。
