Department of Medical Microbiology and Infectious Diseases, Erasmus MC, 's Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands.
Vaccine. 2011 Aug 5;29(34):5603-10. doi: 10.1016/j.vaccine.2011.06.019. Epub 2011 Jun 23.
The primary Moraxella catarrhalis-specific humoral immune response, and its association with nasopharyngeal colonization, was studied in a cohort of infants from birth to 2 years of age. Results indicated that the levels of antigen-specific IgG, IgA and IgM showed extensive inter-individual variability over time, with IgM and IgA levels to all 9 recombinant domains, from 7 different OMPs, being relatively low throughout the study period. In contrast, the level of antigen-specific IgG was significantly higher for the recombinant domains Hag³⁵⁸⁻⁸⁵³, MID⁷⁶⁴⁻⁹¹³, MID⁹⁶²⁻¹²⁰⁰, UspA1⁵⁵⁷⁻⁷⁰⁴ and UspA2¹⁶⁵⁻³¹⁸ in cord blood compared to 6 months of age (P ≤ 0.001). This was a most likely a consequence of maternal transmission of antigen-specific IgG to newborn babies, possibly indicating a future role for these 3 surface antigens in the development of an effective humoral immune response to M. catarrhalis. Finally, at 2 years of age, the levels of antigen-specific IgG still remained far below that obtained from cord blood samples, indicating that the immune response to M. catarrhalis has not matured at 2 years of age. We provide evidence that a humoral antibody response to OMPs UspA1, UspA2 and Hag/MID may play a role in the immune response to community acquired M. catarrhalis colonization events.
本研究旨在探讨婴幼儿从出生到 2 岁期间主要的卡他莫拉菌特异性体液免疫反应及其与鼻咽部定植的关系。结果表明，各抗原特异性 IgG、IgA 和 IgM 水平在整个研究期间随时间呈现广泛的个体间变异性，9 个重组结构域和 7 种不同外膜蛋白（OMP）的所有抗原特异性 IgM 和 IgA 水平均相对较低。相比之下，在脐带血中，重组结构域 Hag358-853、MID764-913、MID962-1200、UspA1557-704 和 UspA2165-318 的抗原特异性 IgG 水平明显高于 6 个月龄时（P≤0.001）。这很可能是由于母体向新生儿传递了抗原特异性 IgG，这可能表明这 3 种表面抗原在卡他莫拉菌产生有效体液免疫反应中具有未来作用。最后，在 2 岁时，抗原特异性 IgG 水平仍远低于脐带血样本，表明卡他莫拉菌的免疫反应在 2 岁时尚未成熟。我们提供的证据表明，OMPs UspA1、UspA2 和 Hag/MID 的体液抗体反应可能在针对社区获得性卡他莫拉菌定植事件的免疫反应中发挥作用。
