Barrera Oro J G, McKee K T, Spisso J, Mahlandt B G, Maiztegui J I
Salk Institute, Government Services Division, Swiftwater, Pennsylvania.
J Virol Methods. 1990 Jul;29(1):71-80. doi: 10.1016/0166-0934(90)90009-5.
A refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against Junin (Argentine hemorrhagic fever) virus. The assay measured neutralizing antibodies after natural as well as vaccine-induced Junin virus infections. Among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. Enhanced sensitivity was achieved by using a standardized complement source (vs human or animal serum) for virus dilution, incubation of virus-serum mixtures at 36 degrees C for 2 h (vs overnight at 4 degrees C) prior to plaque assay, control of age and density of cell monolayers, and variation in overlay conditions.
开发了一种改进的、补体增强的蚀斑减少中和试验，用于检测针对胡宁（阿根廷出血热）病毒的中和抗体。该试验可检测自然感染以及疫苗诱导的胡宁病毒感染后的中和抗体。在接种疫苗的个体中，抗体滴度比传统试验高出2至4倍，且不失特异性。通过使用标准化补体来源（相对于人或动物血清）进行病毒稀释、在蚀斑试验前将病毒 - 血清混合物在36℃孵育2小时（相对于在4℃过夜）、控制细胞单层的年龄和密度以及改变覆盖条件，实现了更高的灵敏度。
