肥胖合并代谢综合征的非糖尿病男性患者减轻体重后对氧磷酶-1 特异性酶活性持续升高。
Persistent elevation of paraoxonase-1 specific enzyme activity after weight reduction in obese non-diabetic men with metabolic syndrome.
机构信息
Cardiovascular Center, Taichung Veterans General Hospital, Taiwan.
出版信息
Clin Chim Acta. 2011 Sep 18;412(19-20):1835-41. doi: 10.1016/j.cca.2011.06.018. Epub 2011 Jun 17.
BACKGROUND
Paraoxonase-1 (PON1) is an esterase associated with the high-density lipoprotein (HDL) in serum. To date, there have been few reports about circulating PON1 protein concentration and specific activity in subjects with metabolic syndrome (MetS). More importantly, it is unknown whether weight loss could alter PON1 protein expression or specific activity in obese non-diabetic men with MetS.
METHODS
We prospectively enrolled a total of 40 obese non-diabetic men with MetS. Among them, 22 subjects finished the 3-month course of weight loss program and complied for longer follow-ups post-weight loss at the 3rd, 12th, and 18th month from the beginning of the program. Twenty-six healthy volunteers served as controls. Serum circulating PON1 concentration was measured by an enzyme linked immunosorbent kit (ELISA) and PON1 activity was measured by an automated PON1 activity assay.
RESULTS
Obese non-diabetic men with MetS (n=40) had a higher PON1 protein concentration (31.0 ± 11.3 vs. 24.8 ± 9.7 μg/ml, p=0.025) but lower specific enzyme activity (7.5 ± 4.0 vs. 11.2 ± 7.2 mU/μg, p=0.023) than those of the controls. Multivariate regression analysis of baseline PON1 specific activity revealed that adiponectin was a significant positive predictor (p=0.044) while monocyte chemotactic protein-1 (MCP-1) was a negative predictor (p=0.031). After a 3-month weight loss program, obese MetS men (n=22) had a significant weight reduction (95.8 ± 9.0 to 86.3 ± 10.4 kg, with a 9.9 ± 5.4% decrease, p<0.001). PON1 protein decreased significantly after weight loss and kept declining through the 3rd month till the 18th month follow-up. PON1 specific enzyme activity (baseline 7.5 ± 2.6 mU/μg) increased significantly after weight loss and kept increasing through the 12th month till the 18th month follow-ups (11.8 ± 6.4 mU/μg, p=0.001 vs. baseline).
CONCLUSIONS
Weight loss by a 3-month diet and exercise program time-sequentially increased PON1 specific enzyme activity in obese non-diabetic men with MetS.
背景
对氧磷酶 1（PON1）是一种与血清高密度脂蛋白（HDL）相关的酯酶。迄今为止，关于代谢综合征（MetS）患者循环 PON1 蛋白浓度和比活性的报道较少。更重要的是，尚不清楚减肥是否会改变肥胖非糖尿病合并 MetS 男性的 PON1 蛋白表达或比活性。
方法
我们前瞻性纳入了 40 名肥胖非糖尿病合并 MetS 的男性患者。其中，22 名患者完成了为期 3 个月的减肥计划，并在减肥开始后的第 3、12 和 18 个月进行了更长时间的随访。26 名健康志愿者作为对照组。通过酶联免疫试剂盒（ELISA）测量血清循环 PON1 浓度，通过自动 PON1 活性测定测量 PON1 活性。
结果
肥胖非糖尿病合并 MetS 的男性（n=40）的 PON1 蛋白浓度（31.0±11.3 比 24.8±9.7μg/ml，p=0.025）较高，但比活性（7.5±4.0 比 11.2±7.2 mU/μg，p=0.023）较低。PON1 比活性的基线多变量回归分析显示，脂联素是一个显著的正预测因子（p=0.044），而单核细胞趋化蛋白-1（MCP-1）是一个负预测因子（p=0.031）。经过 3 个月的减肥计划后，肥胖 MetS 男性（n=22）体重明显减轻（95.8±9.0 至 86.3±10.4kg，减轻 9.9±5.4%，p<0.001）。PON1 蛋白在减肥后显著下降，并在第 3 个月至第 18 个月的随访中持续下降。PON1 比活性（基线 7.5±2.6 mU/μg）在减肥后显著升高，并在第 12 个月至第 18 个月的随访中持续升高（11.8±6.4 mU/μg，p=0.001 比基线）。
结论
通过 3 个月的饮食和运动方案减肥，肥胖非糖尿病合并 MetS 男性的 PON1 比活性依次增加。
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