Unitat de Pediatria, Institut d'Investigació Sanitària Pere Virgili, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
Clin Biochem. 2013 Dec;46(18):1830-6. doi: 10.1016/j.clinbiochem.2013.08.020. Epub 2013 Sep 10.
To investigate the relationships between serum paraoxonase-1 (PON1), insulin resistance, and metabolic syndrome (MetS) in childhood obesity.
We studied 110 obese children and 36 non-obese children with a similar gender and age distribution. We measured serum PON1 activity against 5-thiobutyl butyrolactone (TBBLase) and against paraoxon (paraoxonase). PON1 concentration was measured separately as were the levels of several standard metabolic variables. The homeostasis model assessment (HOMA) index was calculated as an estimate of insulin resistance.
TBBLase was significantly decreased in obese children (P=0.008), while paraoxonase activity and PON1 concentrations showed non-significant trends towards decrease and increase, respectively (P=0.054 and P=0.060). TBBLase and paraoxonase specific activities were significantly decreased (P=0.004 and P=0.018, respectively). TBBLase specific activity was inversely associated with BMI, percentage body fat, insulin, HOMA, triglycerides, and C-reactive protein, and directly associated with HDL-cholesterol. Paraoxonase specific activity showed similar associations with BMI, percentage fat, HDL-cholesterol, and C-reactive protein. Obese children with MetS had lower TBBLase activities than obese children without MetS (P=0.018). Linear regression analyses showed that TBBLase was independently associated with HDL-cholesterol, BMI, percentage body fat and PON155 polymorphism, but paraoxonase activity was associated only with PON1192 polymorphism.
Our results suggest that PON1 may play a role in the onset and development of metabolic alterations in childhood obesity leading to diabetes and cardiovascular disease later in life. However, being derived from statistical association study, this finding cannot be seen as showing cause-effect.
探讨儿童肥胖症患者血清对氧磷酶-1（PON1）、胰岛素抵抗与代谢综合征（MetS）之间的关系。
本研究纳入了 110 名肥胖儿童和 36 名性别、年龄与之相匹配的非肥胖儿童。我们测量了血清中 5-硫代丁基丁酸对硝基苯胺水解酶（TBBLase）和对氧磷酶的活性，同时也分别测量了 PON1 浓度。还测量了多种标准代谢变量的水平。用稳态模型评估（HOMA）指数来评估胰岛素抵抗。
肥胖儿童的 TBBLase 显著降低（P=0.008），而对氧磷酶活性和 PON1 浓度呈下降和升高趋势（P=0.054 和 P=0.060）。TBBLase 和对氧磷酶的比活性均显著降低（P=0.004 和 P=0.018）。TBBLase 比活性与 BMI、体脂百分比、胰岛素、HOMA、甘油三酯和 C 反应蛋白呈负相关，与高密度脂蛋白胆固醇呈正相关。对氧磷酶比活性与 BMI、体脂百分比、HDL-胆固醇和 C 反应蛋白呈相似的相关性。患有 MetS 的肥胖儿童的 TBBLase 活性低于无 MetS 的肥胖儿童（P=0.018）。线性回归分析表明，TBBLase 与 HDL-胆固醇、BMI、体脂百分比和 PON155 多态性独立相关，但对氧磷酶活性仅与 PON1192 多态性相关。
我们的研究结果表明，PON1 可能在儿童肥胖症代谢改变的发生和发展中起作用，导致日后发生糖尿病和心血管疾病。然而，由于这是一项基于统计关联的研究，因此不能将此发现视为因果关系。
