利用 piRNAs 作为标志物检测胃癌患者外周血中的循环肿瘤细胞。
Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using piRNAs as markers.
机构信息
Ningbo University School of Medicine, Ningbo 315211, China.
Ningbo University School of Medicine, Ningbo 315211, China; Affiliated Hospital and Ningbo No. 1 Hospital, Ningbo University School of Medicine, Ningbo 315000, China.
出版信息
Clin Biochem. 2011 Sep;44(13):1050-1057. doi: 10.1016/j.clinbiochem.2011.06.004. Epub 2011 Jun 17.
OBJECTIVE
To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers.
DESIGN AND METHODS
Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in the peripheral blood of 93 patients with gastric cancer and 32 healthy volunteers. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic values.
RESULTS
The peripheral blood levels of piR-651 and piR-823 in the patients with gastric cancer were significantly lower than those from controls (P<0.001). The piR-651 level in gastric adenocarcinoma was higher than that in gastric signet ring cell carcinoma (P = 0.003). The piR-823 level was positively associated with tumor-node-metastasis stage (P = 0.027) and distant metastasis (P = 0.026). The areas under the ROC curve were 0.841, 0.812 and 0.860 for piR-651, piR-823 and the combination, respectively.
CONCLUSIONS
piRNAs may be valuable biomarkers for detecting circulating gastric cancer cells.
目的
探讨利用 Piwi 相互作用 RNA（piRNAs）作为标志物检测胃癌患者外周血循环肿瘤细胞的可行性。
设计与方法
采用实时定量逆转录聚合酶链反应分析 93 例胃癌患者和 32 名健康志愿者外周血中 piR-651 和 piR-823 的水平。构建受试者工作特征（ROC）曲线评估诊断价值。
结果
胃癌患者外周血 piR-651 和 piR-823 水平明显低于对照组（P<0.001）。胃腺癌患者的 piR-651 水平高于胃印戒细胞癌（P=0.003）。piR-823 水平与肿瘤-淋巴结-转移分期（P=0.027）和远处转移（P=0.026）呈正相关。piR-651、piR-823 和联合检测的 ROC 曲线下面积分别为 0.841、0.812 和 0.860。
结论
piRNAs 可能是检测循环胃癌细胞的有价值的生物标志物。
Zotero 插件