Department of Molecular Biology, Graduate School of Pharmaceutical Science, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi, Japan.
Exp Cell Res. 2011 Sep 10;317(15):2110-23. doi: 10.1016/j.yexcr.2011.06.003. Epub 2011 Jun 17.
Factor for adipocyte differentiation 104 (fad104) is a regulator of adipogenesis and osteogenesis. Our previous study showed that fad104-deficient mice died immediately after birth, suggesting fad104 to be essential for neonatal survival. However, the cause of this rapid death is unclear. Here, we demonstrate the role of fad104 in neonatal survival. Phenotypic and morphological analyses showed that fad104-deficient mice died due to cyanosis-associated lung dysplasia including atelectasis. Furthermore, immunohistochemistry revealed that FAD104 was strongly expressed in ATII cells in the developing lung. Most importantly, the ATII cells in lungs were immature, and impaired the expression of surfactant-associated proteins. Collectively, these results indicate that fad104 has an indispensable role in lung maturation, especially the maturation and differentiation of ATII cells.
脂肪细胞分化因子 104（fad104）是脂肪生成和骨生成的调节剂。我们之前的研究表明 fad104 缺陷型小鼠在出生后立即死亡，表明 fad104 对新生儿的存活至关重要。然而，这种快速死亡的原因尚不清楚。在这里，我们证明了 fad104 在新生儿存活中的作用。表型和形态分析表明， fad104 缺陷型小鼠因发绀相关的肺发育不良（包括肺不张）而死亡。此外，免疫组织化学显示 FAD104 在发育中的肺的 ATII 细胞中强烈表达。最重要的是，肺中的 ATII 细胞不成熟，并损害了表面活性剂相关蛋白的表达。总之，这些结果表明 fad104 在肺成熟中具有不可或缺的作用，特别是 ATII 细胞的成熟和分化。
