Huang Si-Yang, Zhao Guang-Hui, Fu Bao-Quan, Xu Min-Jun, Wang Chun-Ren, Wu Song-Ming, Zou Feng-Cai, Zhu Xing-Quan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province 730046, China.
Parasitol Int. 2012 Mar;61(1):71-6. doi: 10.1016/j.parint.2011.06.008. Epub 2011 Jun 17.
Clonorchiasis caused by Clonorchis sinensis is an important foodborne parasitosis of humans and animals, and is predominantly a hepatobiliary disease. Globally, nearly 35 million people were infected with C. sinensis, with approximately 15 million being in China. Patients would chronically present fatigue, jaundice, abdominal discomfort, along with the increased risk of developing into a form of cholangiocarcinoma that is fatal to humans. Treatment of clonorchiasis by praziquantel has been very successful, but this is dependent on early accurate diagnosis and correct species identification. The present article reviews the current status of knowledge in genomics and functional genomics of C. sinensis, and summarizes the main DNA-based techniques for the specific diagnosis of C. sinensis infection and studies of genetic variation in C. sinensis, and provides perspectives for future studies. The advances in genomics and molecular genetics of C. sinensis shed new sight on our understanding of population structure of C. sinensis as well as the prevention and control of clonorchiasis.
华支睾吸虫引起的华支睾吸虫病是人和动物重要的食源性寄生虫病，主要是一种肝胆疾病。在全球范围内，近3500万人感染华支睾吸虫，其中约1500万人在中国。患者会长期出现疲劳、黄疸、腹部不适，同时发展为对人类致命的胆管癌的风险增加。用吡喹酮治疗华支睾吸虫病非常成功，但这取决于早期准确诊断和正确的物种鉴定。本文综述了华支睾吸虫基因组学和功能基因组学的研究现状，总结了基于DNA的华支睾吸虫感染特异性诊断和华支睾吸虫遗传变异研究的主要技术，并为未来研究提供了展望。华支睾吸虫基因组学和分子遗传学的进展为我们理解华支睾吸虫的种群结构以及华支睾吸虫病的预防和控制提供了新的视角。
