Zuba-Surma Ewa K, Ratajczak Mariusz Z
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Methods Cell Biol. 2011;102:207-30. doi: 10.1016/B978-0-12-374912-3.00008-0.
Imaging cytometry has recently become an important achievement in development of flow cytometric technologies. The ImageStream cytometer combines the vast features of classical flow cytometry including an impartial analysis of great number of cells in short period of time which results in strong statistical data output, with essential features of fluorescence microscopy such us collecting of real multiparameter images of analyzed objects. In this chapter, we would like to introduce an overview of imaging cytometry platform and emphasize the potential advantages of using this system for several experimental purposes. Moreover, both well established as well as potential applications of imaging cytometry will be described. Eventually, we would like to illustrate the unique use of ImageStream cytometer for identification and characterization of subpopulations of stem/ progenitor cells present in different biological specimens.
成像细胞术最近已成为流式细胞术技术发展中的一项重要成果。ImageStream 细胞仪结合了经典流式细胞术的众多特性，包括在短时间内对大量细胞进行客观分析从而产生强大的统计数据输出，以及荧光显微镜的基本特性，如收集被分析对象的真实多参数图像。在本章中，我们将介绍成像细胞术平台的概述，并强调将该系统用于多种实验目的的潜在优势。此外，还将描述成像细胞术已确立的应用以及潜在应用。最后，我们将举例说明 ImageStream 细胞仪在鉴定和表征存在于不同生物样本中的干/祖细胞亚群方面的独特用途。
