Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA.
Opt Lett. 2012 Feb 15;37(4):707-9. doi: 10.1364/OL.37.000707.
This letter introduces a fluidics-based focus-stack collecting microscope. A microfluidic device transports cells through the focal plane of a microscope, resulting in an efficient method to collect focus stacks of large collections of single cells. Images from the focus stacks are used to reconstruct the quantitative phase of cells with the transport-of-intensity-equation method. Using the phase imaging flow cytometer, we measure three-dimensional shape variations of red blood and leukemia cells.
这封信介绍了一种基于流控技术的聚焦堆叠采集显微镜。微流控装置将细胞输送通过显微镜的焦平面，从而有效地收集大量单细胞的聚焦堆叠。从聚焦堆叠图像中，我们使用强度传输方程方法重建细胞的定量相位。使用相成像流式细胞仪，我们测量红细胞和白血病细胞的三维形状变化。
