流式细胞术中的激光

Lasers in flow cytometry.

作者信息

Telford William G

机构信息

Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

出版信息

Methods Cell Biol. 2011;102:375-409. doi: 10.1016/B978-0-12-374912-3.00015-8.

DOI:10.1016/B978-0-12-374912-3.00015-8

PMID:21704847

Abstract

Laser technology has advanced tremendously since the first gas lasers were incorporated into early flow cytometers. Gas lasers have been largely replaced by solid-state laser technology, making virtually any desirable visible light wavelength available for flow cytometry. Multiwavelength, white light, and wavelength tunable lasers are poised to enhance our analytical capabilities even further. In this chapter, I summarize the role that lasers play in cytometry, and the practical characteristics that make a laser appropriate for flow cytometry. I then review the latest single wavelength lasers available for flow cytometry, and how they can be used to excite the ever-expanding array of available fluorochromes. Finally, I review the contribution and potential of the latest tunable laser technology to flow cytometry, and show several examples of these novel sources integrated into production instruments. Technical details and critical parameters for successful application of these lasers for biomedical analysis are covered in depth.

摘要

自第一台气体激光器被应用于早期流式细胞仪以来，激光技术取得了巨大进步。气体激光器在很大程度上已被固态激光技术所取代，这使得几乎任何所需的可见光波长都可用于流式细胞术。多波长、白光和波长可调谐激光器有望进一步提升我们的分析能力。在本章中，我总结了激光在细胞计数中的作用，以及使激光适用于流式细胞术的实际特性。然后，我回顾了目前可用于流式细胞术的最新单波长激光器，以及它们如何用于激发种类不断增加的可用荧光染料。最后，我回顾了最新可调谐激光技术对流式细胞术的贡献和潜力，并展示了这些新型光源集成到生产仪器中的几个实例。文中深入探讨了这些激光器在生物医学分析中成功应用的技术细节和关键参数。