流式细胞术中的小型激光器。

Small lasers in flow cytometry.

作者信息

Telford William G

机构信息

Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2004;263:399-418. doi: 10.1385/1-59259-773-4:399.

DOI:10.1385/1-59259-773-4:399

PMID:14976380

Abstract

Laser technology has made tremendous advances in recent years, particularly in the area of diode and diode-pumped solid state sources. Flow cytometry has been a direct beneficiary of these advances, as these small, low-maintenance, inexpensive lasers with reasonable power outputs are integrated into flow cytometers. In this chapter we review the contribution and potential of solid-state lasers to flow cytometry, and show several examples of these novel sources integrated into production flow cytometers. Technical details and critical parameters for successful application of these lasers for biomedical analysis are reviewed.

摘要

近年来，激光技术取得了巨大进展，尤其是在二极管和二极管泵浦固态光源领域。流式细胞术直接受益于这些进展，因为这些体积小、维护需求低、价格低廉且功率输出合理的激光器被集成到流式细胞仪中。在本章中，我们回顾了固态激光器对流式细胞术的贡献和潜力，并展示了这些新型光源集成到商用流式细胞仪中的几个实例。还回顾了将这些激光器成功应用于生物医学分析的技术细节和关键参数。