流式细胞术用激光概述

Overview of Lasers for Flow Cytometry.

作者信息

Telford William G

机构信息

Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Building 10-CRC Room 3-3297 East, 10 Center Drive, Bethesda, MD, 20892, USA.

出版信息

Methods Mol Biol. 2018;1678:447-479. doi: 10.1007/978-1-4939-7346-0_19.

DOI:10.1007/978-1-4939-7346-0_19

PMID:29071690

Abstract

Lasers are critical elements of all flow cytometers. Instrument capabilities are dictated by the wavelengths and characteristics of its laser sources. In this chapter, we review the lasers available for flow cytometry, and provide guidance in choosing laser wavelengths and characteristics to best match the analytical needs of biomedical research laboratories.

摘要

激光是所有流式细胞仪的关键部件。仪器的性能取决于其激光源的波长和特性。在本章中，我们将回顾可用于流式细胞术的激光，并为选择最能满足生物医学研究实验室分析需求的激光波长和特性提供指导。