Otsuka Y, Nagano K, Nagano K, Hori K, Oh-ishi J, Hayashi H, Watanabe N, Niitsu Y
Pharmaceuticals Laboratory, Asahi Chemical Industry Co. Ltd., Fuji, Japan.
J Immunol. 1990 Oct 15;145(8):2639-43.
Coincubation of neutrophils with TNF inhibited the chemoattractant-directed migration of neutrophils under agarose and enhanced their migration in the multiwell chemotaxis chamber. To assess the physiological significance of these differing in vitro TNF effects, ex vivo and in vivo investigations were performed using animal models. Neutrophils from the peripheral blood of rabbits preadministered systemic TNF showed impaired ability to migrate toward chemoattractants in vitro. In addition, systemic TNF administration suppressed zymosan-activated plasma-induced local accumulation of leukocytes in mouse skin. The results indicate that circulating TNF may act as a suppressor for local inflammatory reaction.
中性粒细胞与肿瘤坏死因子（TNF）共同孵育会抑制中性粒细胞在琼脂糖下的趋化因子导向迁移，并增强它们在多孔趋化性小室中的迁移。为了评估这些不同的体外TNF效应的生理意义，使用动物模型进行了体内外研究。预先全身性给予TNF的兔外周血中的中性粒细胞在体外向趋化因子迁移的能力受损。此外，全身性给予TNF会抑制酵母聚糖激活的血浆诱导的小鼠皮肤中白细胞的局部聚集。结果表明，循环中的TNF可能作为局部炎症反应的抑制剂。
