Department of Ophthalmology and Research Institute of Medical Sciences, Chonnam National University Medical School and Hospital, Gwangju, Korea.
Cornea. 2011 Sep;30(9):972-6. doi: 10.1097/ICO.0b013e31820687dd.
To determine the relative staining characteristics of the nasal and temporal conjunctiva as compared with other measures of dry eye and evaluate the qualitative clinical impression of the utility of the double vital staining with fluorescein and lissamine green.
Ocular surface staining was performed with a mixture of 1% fluorescein and 1% lissamine green in 50 patients with dry eye (12 patients with Sjögren syndrome and 38 patients with non-Sjögren syndrome). Ocular surface disease index (OSDI), tear breakup time (BUT), and Schirmer test were evaluated. Digital photographs were taken after vital staining on the ocular surface, and the grade of staining in various areas was assessed. Correlation among the degrees of staining, OSDI, and ocular surface parameters was analyzed.
The nasal conjunctiva evidenced greater staining compared with the temporal conjunctiva and cornea (P = 0.04). Staining of the nasal conjunctiva showed significant correlation with OSDI (P < 0.01) and BUT (P = 0.03). Staining of the temporal conjunctiva correlated significantly with OSDI (P = 0.01), and staining of the cornea correlated with BUT (P = 0.02).
Double vital staining with 1% fluorescein and 1% lissamine green correlates with symptoms and some ocular surface parameters in patients with dry eye and helps to identify ocular surface changes easily. It may be a useful method for the diagnosis of dry eye and the assessment of the therapeutic effect in patients with dry eye syndrome.
比较鼻侧和颞侧结膜的相对染色特征与其他干眼症测量指标，并评估双荧光素和丽丝胺绿双重活染色的临床应用效果。
对 50 例干眼症患者（12 例干燥综合征患者和 38 例非干燥综合征患者）进行混合 1%荧光素和 1%丽丝胺绿的眼表面染色。评估眼表面疾病指数（OSDI）、泪膜破裂时间（BUT）和 Schirmer 测试。在眼表面活染色后拍摄数字照片，并评估各个区域的染色等级。分析染色程度与 OSDI 和眼表面参数之间的相关性。
与颞侧和角膜相比，鼻侧结膜的染色程度更明显（P = 0.04）。鼻侧结膜的染色与 OSDI（P < 0.01）和 BUT（P = 0.03）呈显著相关性。颞侧结膜的染色与 OSDI 呈显著相关性（P = 0.01），角膜的染色与 BUT 呈相关性（P = 0.02）。
1%荧光素和 1%丽丝胺绿的双重活染色与干眼症患者的症状和一些眼表面参数相关，有助于轻松识别眼表面变化。它可能是诊断干眼症和评估干眼症综合征患者治疗效果的有用方法。
