National Center of Excellence for Environmental and Hazardous Waste Management, Chulalongkorn University, Bangkok, Thailand.
Microb Ecol. 2011 Nov;62(4):776-88. doi: 10.1007/s00248-011-9893-9. Epub 2011 Jun 25.
The contribution of ammonia-oxidizing archaea (AOA) to nitrogen removal in wastewater treatment plants (WWTPs) remains unknown. This study investigated the abundance of archaeal (AOA) and bacterial (ammonia-oxidizing bacteria (AOB)) amoA genes in eight of Bangkok's municipal WWTPs. AOA amoA genes (3.28 × 10(7) ± 1.74 × 10(7)-2.23 × 10(11) ± 1.92 × 10(11) copies l(-1) sludge) outnumbered AOB amoA genes in most of the WWTPs even though the plants' treatment processes, influent and effluent characteristics, removal efficiencies, and operation varied. An estimation of the ammonia-oxidizing activity of AOA and AOB suggests that AOA involved in autotrophic ammonia oxidation in the WWTPs. Statistical analysis shows that the numbers of AOA amoA genes correlated negatively to the ammonium levels in effluent wastewater, while no correlation was found between the AOA amoA gene numbers and the oxygen concentrations in aeration tanks. An analysis of the AOB sequences shows that AOB found in the WWTPs limited to only two AOB clusters which exhibit high or moderate affinity to ammonia. In contrast to AOB, AOA sequences of various clusters were retrieved, and they were previously recovered from a variety of environments, such as thermal and marine environments.
氨氧化古菌（AOA）对污水处理厂（WWTP）中氮去除的贡献仍不清楚。本研究调查了曼谷 8 个城市 WWTP 中古菌（AOA）和细菌（氨氧化细菌（AOB））amoA 基因的丰度。尽管这些工厂的处理过程、进水和出水特性、去除效率和运行情况各不相同，但大多数 WWTP 中的 AOA amoA 基因数量（3.28×10(7)±1.74×10(7)-2.23×10(11)±1.92×10(11)拷贝/克污泥）超过 AOB amoA 基因。对 AOA 和 AOB 的氨氧化活性的估计表明，AOA 参与了 WWTP 中的自养氨氧化。统计分析表明，AOA amoA 基因数量与废水出水的氨氮水平呈负相关，而 AOA amoA 基因数量与曝气池中的氧浓度之间没有相关性。AOB 序列分析表明，WWTP 中发现的 AOB 仅限于仅对氨具有高或中等亲和力的两个 AOB 聚类。与 AOB 不同，各种聚类的 AOA 序列被检索到，并且它们之前从各种环境中回收，例如热和海洋环境。
