College of Life Sciences, Hebei University, 071002 Baoding, People's Republic of China.
Mol Biol Rep. 2012 Mar;39(3):3305-10. doi: 10.1007/s11033-011-1099-1. Epub 2011 Jun 26.
Hemocyanins are copper-containing (Cu(+)) proteins that transport oxygen in many arthropods hemolymph. We characterized Hc1 gene from the grasshopper species Locusta migratoria manilensis. In particular, we cloned and sequenced the corresponding cDNAs and studied their expression at different developmental stages. The cDNA of Hc1 gene (GenBank accession no.:HQ213937) is 2271 bp in length and the open reading frame is 2016 bp, which encodes a 672 amino acids protein with a calculated molecular mass of 77.9 kD and the isoelectric point of 6.06. Sequence alignment analysis result showed that this gene shares 94.7% identity with Schistocerca americana EHP. In addition, analysis of quantitative RT-PCR indicated that, LmiHc1 was expressed in the embyro (24, 39, 62, 86, 144, and 193 h after hatch), nymphs (1st instar, 2nd instar, 3rd instar, 4th instar and 5th instar) and in adult. These results showed that Hc1 plays an important role in grasshopper, which may be related to an enhanced oxygen supply. Phylogenetic analysis of insecta based on Hc1 are basically consistent with the morphology.
血蓝蛋白是一种含铜（Cu(+））的蛋白质，在许多节肢动物的血淋巴中运输氧气。我们对东亚飞蝗的血蓝蛋白基因 Hc1 进行了鉴定。特别地，我们克隆并测序了相应的 cDNA，并研究了它们在不同发育阶段的表达情况。Hc1 基因的 cDNA（GenBank 登录号：HQ213937）长 2271 个碱基对，开放阅读框为 2016 个碱基对，编码一个 672 个氨基酸的蛋白质，计算分子量为 77.9 kD，等电点为 6.06。序列比对分析结果表明，该基因与美洲大蠊 EHP 的同源性为 94.7%。此外，定量 RT-PCR 分析表明，LmiHc1 在胚胎（孵化后 24、39、62、86、144 和 193 小时）、若虫（1 龄、2 龄、3 龄、4 龄和 5 龄）和成体中均有表达。这些结果表明 Hc1 在蝗虫中发挥着重要作用，这可能与增强氧气供应有关。基于 Hc1 的昆虫系统发育分析与形态学基本一致。
