Nicholas School of Environment, Duke University, Box 90328, Durham, NC 27708-0328, USA.
Ecotoxicology. 2011 Nov;20(8):1890-9. doi: 10.1007/s10646-011-0727-9. Epub 2011 Jun 26.
The Atlantic Wood Industries Superfund site (AWI) on the Elizabeth River in Portsmouth, VA is heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) from a wood treatment facility. Atlantic killifish, or mummichog (Fundulus heteroclitus), at this Superfund site are exposed to very high concentrations of several carcinogens. In this study, we measured PAH concentrations in both fish tissues and sediments. Concurrently, we assessed different aspects of genotoxicity in the killifish exposed in situ. Both sediment and tissue PAH levels were significantly higher in AWI samples, relative to a reference site, but the chemistry profile was different between sediments and tissues. Killifish at AWI exhibited higher levels of DNA damage compared to reference fish, as measured via the flow cytometric method (FCM), and the damage was consistent with sediment PAH concentrations. Covalent binding of benzo[a]pyrene (BaP) metabolites to DNA, as measured via LC-MS/MS adduct detection methods, were also elevated and could be partially responsible for the DNA damage. Using similar LC-MS/MS methods, we found no evidence that oxidative DNA adducts had a role in observed genotoxicity.
弗吉尼亚州朴茨茅斯伊丽莎白河上的大西洋木材工业超级基金场地（AWI）受到木材处理设施的多环芳烃（PAHs）的严重污染。在这个超级基金场地，大西洋锯脂鲤（Fundulus heteroclitus）或石首鱼，暴露在几种致癌物质的极高浓度下。在这项研究中，我们测量了鱼类组织和沉积物中的 PAH 浓度。同时，我们评估了现场暴露的锯脂鲤的不同遗传毒性方面。与参考地点相比，AWI 样本中的沉积物和组织 PAH 水平显著更高，但沉积物和组织之间的化学特征不同。与参考鱼类相比，通过流式细胞仪方法（FCM）测量，AWI 中的锯脂鲤表现出更高水平的 DNA 损伤，而且这种损伤与沉积物 PAH 浓度一致。通过 LC-MS/MS 加合物检测方法测量的苯并[a]芘（BaP）代谢物与 DNA 的共价结合也升高，这可能是导致 DNA 损伤的部分原因。使用类似的 LC-MS/MS 方法，我们没有发现氧化 DNA 加合物在观察到的遗传毒性中起作用的证据。
