Aulasevich Alena, Roskamp Robert F, Jonas Ulrich, Menges Bernhard, Dostálek Jakub, Knoll Wolfgang
Max-Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
Macromol Rapid Commun. 2009 May 19;30(9-10):872-7. doi: 10.1002/marc.200800747. Epub 2009 Mar 17.
This article reports the implementation of optical waveguide spectroscopy (OWS) for the quantitative time-resolved observation of changes in the swelling behavior and mass density of protein-functionalized hydrogel films. In the experiment, a thin film of an N-isopropylacrylamide (NIPAAm)-based polymer that supported optical waveguide modes is attached to a metallic sensor surface. IgG molecules are in situ immobilized in this gel by using novel coupling chemistry with a charge-attraction scheme based on a tetrafluorophenol sulfonate active ester. The anti-fouling properties of the functionalized hydrogel network and the kinetics of the affinity binding of protein molecules in the gel are investigated.
本文报道了利用光波导光谱法（OWS）对蛋白质功能化水凝胶薄膜的溶胀行为和质量密度变化进行定量时间分辨观测的研究。实验中，将一种支持光波导模式的基于N-异丙基丙烯酰胺（NIPAAm）的聚合物薄膜附着在金属传感器表面。通过使用基于四氟苯酚磺酸盐活性酯的电荷吸引方案的新型偶联化学方法，将IgG分子原位固定在该凝胶中。研究了功能化水凝胶网络的抗污性能以及凝胶中蛋白质分子的亲和结合动力学。
