Garcia-Vallve Santiago
Departament de Bioquímica i Biotecnologia. Facultat de Química, Universitat Rovira i Virgili, 43005 Tarragona, Spain.
Biochem Mol Biol Educ. 2004 Jan;32(1):17-9. doi: 10.1002/bmb.2004.494032010308.
A new parameter is presented for considering the contribution from each complex of the mitochondrial respiratory chain to the creation of the electrochemical gradient. This parameter is the proton gradient generated in each complex per pair of electrons transported (ΔH(+) /2e(-) ) and may be calculated as the sum of the electrons taken up from the N-side and the electrons released at the P-side. The ΔH(+) /2e(-) values take into account all the electrons taken up and released in each complex and differentiate between the contribution from protons that are translocated from the N-side to the P-side and the contribution of scalar protons. Using these values, the P/O ratios for the oxidation of NADH and succinate are predicted to be 2.5 and 1.5, respectively.
提出了一个新参数，用于考量线粒体呼吸链各复合体对电化学梯度形成的贡献。该参数是每对电子传递时各复合体中产生的质子梯度（ΔH(+) /2e(-) ），可计算为从N侧摄取的电子与在P侧释放的电子之和。ΔH(+) /2e(-) 值考虑了各复合体中摄取和释放的所有电子，并区分了从N侧转运至P侧的质子贡献和标量质子的贡献。利用这些值，预测NADH和琥珀酸氧化的P/O比值分别为2.5和1.5。
