Curtright Robert, Emry Randall, Heaton Ruth M, Markwell John
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664.
Biochem Mol Biol Educ. 2004 Mar;32(2):71-7. doi: 10.1002/bmb.2004.494032020314.
We describe a simple undergraduate exercise involving the titration of a weak acid by a strong base using a pH meter and a micropipette. Students then use their data and carry out graphical analyses with a spreadsheet. The analyses involve using mathematical concepts such as first-derivative and semi-log plots and provide an opportunity for collaboration between biochemistry and mathematics instructors. By focusing on titration data, rather than the titration process, and using a variety of graphical transformations, we believe that students achieve a deeper understanding of the concept of buffering.
我们描述了一个简单的本科实验，该实验涉及使用pH计和微量移液器用强碱滴定弱酸。然后学生们利用他们的数据，并用电子表格进行图形分析。这些分析涉及使用诸如一阶导数和半对数图等数学概念，并为生物化学和数学教师之间的合作提供了机会。通过关注滴定数据而非滴定过程，并使用各种图形变换，我们相信学生能对缓冲概念有更深入的理解。
