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Tagging and purifying proteins to teach molecular biology and advanced biochemistry.

作者信息

Roecklein-Canfield Jennifer A, Lopilato Jane

机构信息

Departments of Chemistry, Boston, Massachusetts 02115.

出版信息

Biochem Mol Biol Educ. 2004 Nov;32(6):373-7. doi: 10.1002/bmb.2004.494032060399.

Abstract

Two distinct courses, "Molecular Biology" taught by the Biology Department and "Advanced Biochemistry" taught by the Chemistry Department, complement each other and, when taught in a coordinated and integrated way, can enhance student learning and understanding of complex material. "Molecular Biology" is a comprehensive lecture-based course with a 3-h laboratory once a week, while "Advanced Biochemistry" is a completely laboratory-based course with lecture fully integrated around independent student projects. Both courses emphasize and utilize cutting-edge technology. Teaching across departmental boundaries allows students access to faculty expertise and techniques rarely used at the undergraduate level, namely the tagging of proteins and their use in protein purification.

摘要

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