Cognitive Tools and Data Management Department, The MITRE Corporation, Bedford, MA, USA.
Lett Appl Microbiol. 2011 Sep;53(3):306-12. doi: 10.1111/j.1472-765X.2011.03107.x. Epub 2011 Jul 14.
To evaluate the feasibility of identifying viruses from aircraft cabin air, we evaluated whether respiratory viruses trapped by commercial aircraft air filters can be extracted and detected using a multiplex PCR, bead-based assay.
The ResPlex II assay was first tested for its ability to detect inactivated viruses applied to new filter material; all 18 applications of virus at a high concentration were detected. The ResPlex II assay was then used to test for 18 respiratory viruses on 48 used air filter samples from commercial aircraft. Three samples tested positive for viruses, and three viruses were detected: rhinovirus, influenza A and influenza B. For 33 of 48 samples, internal PCR controls performed suboptimally, suggesting sample matrix effect.
In some cases, influenza and rhinovirus RNA can be detected on aircraft air filters, even more than 10 days after the filters were removed from aircraft.
With protocol modifications to overcome PCR inhibition, air filter sampling and the ResPlex II assay could be used to characterize viruses in aircraft cabin air. Information about viruses in aircraft could support public health measures to reduce disease transmission within aircraft and between cities.
评估从飞机机舱空气中鉴定病毒的可行性，我们评估了商用飞机空气过滤器捕获的呼吸道病毒是否可以使用多重 PCR、基于珠子的检测方法提取和检测。
首先测试 ResPlex II 检测方法对应用于新过滤材料的灭活病毒的检测能力；高浓度病毒的 18 次应用均被检测到。然后，使用 ResPlex II 检测方法对来自商用飞机的 48 个用过的空气过滤器样本中的 18 种呼吸道病毒进行检测。有三个样本的病毒检测呈阳性，检测到三种病毒：鼻病毒、甲型流感病毒和乙型流感病毒。在 48 个样本中的 33 个样本中，内部 PCR 对照的性能不理想，表明样本基质效应。
在某些情况下，甚至在过滤器从飞机上取下 10 多天后，仍可在飞机空气过滤器上检测到流感病毒和鼻病毒 RNA。
通过修改方案以克服 PCR 抑制，空气过滤器采样和 ResPlex II 检测方法可用于描述飞机机舱空气中的病毒。有关飞机中病毒的信息可以支持公共卫生措施，以减少飞机内和城市之间的疾病传播。
