Department of Dermatology, Third People's Hospital of Hangzhou, Hangzhou, China.
Skin Res Technol. 2011 Nov;17(4):505-9. doi: 10.1111/j.1600-0846.2011.00525.x. Epub 2011 Jun 28.
Nonablative laser has been used for the treatment and prevention of skin aging for many years. Although the mechanism has not been elucidated, histological evaluation showed that the dermal fibroblasts and its collagen production should be the main target for this rejuvenation. In order to determine the effects of a 1320 nm nonablative laser on the human dermal fibroblasts, the two main secretion factors, basic fibroblast growth factor (bFGF) and transforming growth factor β1(TGF-β1), in vitro were detected.
The human dermal fibroblasts were cultured and irradiated with a 1320 nm laser at the dose of 15, 20, and 24 J/cm(2) respectively. The number of fibroblasts was counted and the levels of bFGF and TGF-β1 were detected by enzyme-linked immunosorbent assay at the time points of 0, 24, 48, and 72 h after irradiation.
The results showed that both the number of fibroblasts and the secretion of bFGF increased after the irradiation at the dose of 20 and 24 J/cm(2) (P<0.05) compared with that of the control cells. The bFGF secretion in the group exposed to 20 J/cm(2) was more significant than that of 24 J/cm(2), and the peak level was 24 h after irradiation. The level of TGF-β1 secretion decreased after irradiation in a dose-dependent manner (15 and 20 J/cm(2), both P<0.05; 24 J/cm(2), P<0.01), and reached a nadir at 24 h.
Our results suggested that the 1320 nm nonablative laser accelerates the vitality of fibroblasts, promotes the secretion of bFGF, and inhibits TGF-β1 secretion by fibroblasts.
非剥脱性激光已被用于治疗和预防皮肤老化多年。虽然其机制尚未阐明，但组织学评估表明，真皮成纤维细胞及其胶原产生应该是这种年轻化的主要目标。为了确定 1320nm 非剥脱性激光对人真皮成纤维细胞的影响，体外检测了两种主要的分泌因子，碱性成纤维细胞生长因子（bFGF）和转化生长因子β1（TGF-β1）。
培养人真皮成纤维细胞，用 1320nm 激光分别照射 15、20 和 24J/cm2，照射后 0、24、48 和 72h 时用酶联免疫吸附法检测成纤维细胞数量和 bFGF、TGF-β1 水平。
结果显示，与对照组相比，照射剂量为 20 和 24J/cm2 时，成纤维细胞数量和 bFGF 分泌均增加（P<0.05）。20J/cm2 照射组的 bFGF 分泌更为显著，其峰值水平出现在照射后 24h。TGF-β1 分泌随照射剂量呈剂量依赖性下降（15 和 20J/cm2，均 P<0.05；24J/cm2，P<0.01），并在 24h 时达到最低值。
我们的结果表明，1320nm 非剥脱性激光加速了成纤维细胞的活力，促进了 bFGF 的分泌，并抑制了成纤维细胞 TGF-β1 的分泌。
