Molecular Ecology Group, Institute of Ecology, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria.
Mol Ecol Resour. 2011 Nov;11(6):1113-8. doi: 10.1111/j.1755-0998.2011.03043.x. Epub 2011 Jun 28.
Amplified fragment length polymorphism (AFLP), a widely used method for DNA fingerprinting, has shifted from polyacrylamide gel to capillary electrophoresis over the last years. Currently, most AFLP data are generated in a computer-readable format, and several programs are available that automatically score raw data into binary profiles. Good scoring parameters are the key to good AFLP profiles. optiFLP is the first open source program for automatic optimization of AFLP scoring parameters. It searches parameter space to maximize the contrast among groups of AFLP profiles, with the allocation of profiles to groups in either a supervised or an unsupervised mode. The software produces output files ready for use in a range of downstream applications.
扩增片段长度多态性 (AFLP) 是一种广泛用于 DNA 指纹图谱分析的方法，近年来已从聚丙烯酰胺凝胶电泳转向毛细管电泳。目前，大多数 AFLP 数据以计算机可读的格式生成，并且有几个程序可用于将原始数据自动评分成二进制图谱。良好的评分参数是 AFLP 图谱质量的关键。optiFLP 是第一个用于 AFLP 评分参数自动优化的开源程序。它在参数空间中搜索，以最大化 AFLP 图谱组之间的对比度，并且以有监督或无监督的模式将图谱分配到组中。该软件生成可用于一系列下游应用的输出文件。
