Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58108, USA.
BMC Microbiol. 2011 Jun 27;11:153. doi: 10.1186/1471-2180-11-153.
Salmonella species are recognized worldwide as a significant cause of human and animal disease. In this study the molecular profiles and characteristics of Salmonella enterica Senftenberg isolated from human cases of illness and those recovered from healthy or diagnostic cases in animals were assessed. Included in the study was a comparison with our own sequenced strain of S. Senfteberg recovered from production turkeys in North Dakota. Isolates examined in this study were subjected to antimicrobial susceptibility profiling using the National Antimicrobial Resistance Monitoring System (NARMS) panel which tested susceptibility to 15 different antimicrobial agents. The molecular profiles of all isolates were determined using Pulsed Field Gel Electrophoresis (PFGE) and the sequence types of the strains were obtained using Multi-Locus Sequence Type (MLST) analysis based on amplification and sequence interrogation of seven housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA, and thrA). PFGE data was input into BioNumerics analysis software to generate a dendrogram of relatedness among the strains.
The study found 93 profiles among 98 S. Senftenberg isolates tested and there were primarily two sequence types associated with humans and animals (ST185 and ST14) with overlap observed in all host types suggesting that the distribution of S. Senftenberg sequence types is not host dependent. Antimicrobial resistance was observed among the animal strains, however no resistance was detected in human isolates suggesting that animal husbandry has a significant influence on the selection and promotion of antimicrobial resistance.
The data demonstrates the circulation of at least two strain types in both animal and human health suggesting that S. Senftenberg is relatively homogeneous in its distribution. The data generated in this study could be used towards defining a pathotype for this serovar.
沙门氏菌属在世界范围内被公认为人类和动物疾病的重要病因。在这项研究中，评估了从人类疾病和动物健康或诊断病例中分离出的肠炎沙门氏菌的分子特征。研究中还包括与我们从北达科他州生产火鸡中分离出的自己测序的肠炎沙门氏菌菌株进行比较。本研究中检查的分离株使用国家抗菌药物耐药性监测系统（NARMS）面板进行了抗菌药物敏感性分析，该面板测试了对 15 种不同抗菌药物的敏感性。所有分离株的分子特征均通过脉冲场凝胶电泳（PFGE）确定，菌株的序列类型通过基于扩增和七个管家基因（aroC、dnaN、hemD、hisD、purE、sucA 和 thrA）序列询问的多位点序列类型（MLST）分析获得。PFGE 数据输入到 BioNumerics 分析软件中，以生成菌株之间亲缘关系的聚类图。
该研究在 98 株肠炎沙门氏菌分离株中发现了 93 种图谱，主要有两种与人类和动物相关的序列类型（ST185 和 ST14），在所有宿主类型中均观察到重叠，表明肠炎沙门氏菌序列类型的分布不受宿主的影响。在动物菌株中观察到了抗菌药物耐药性，但在人类分离株中未检测到耐药性，这表明畜牧业对选择和促进抗菌药物耐药性有重大影响。
数据表明至少有两种菌株类型在动物和人类健康中循环，这表明肠炎沙门氏菌在其分布上相对均匀。本研究产生的数据可用于定义该血清型的病原体。
