利用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）对马来西亚鸡肉及鸡肉制品中分离出的肠炎沙门氏菌肠炎血清型菌株进行分析。
Analysis of Salmonella enterica serovar Enteritidis isolates from chickens and chicken meat products in Malaysia using PFGE, and MLST.
作者信息
Zakaria Zunita, Hassan Latiffah, Sharif Zawiyah, Ahmad Norazah, Ali Rohaya Mohd, Husin Suraya Amir, Hazis Nor Hazrin Binti Abd, Sohaimi Nor Fitriah Mohamed, Bakar Shafini Abu, Garba Bashiru
机构信息
Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Bacteriology Laboratory, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
出版信息
BMC Vet Res. 2020 Oct 17;16(1):393. doi: 10.1186/s12917-020-02605-y.
BACKGROUND
Salmonella is a very important foodborne pathogen causing illness in humans. The emergence of drug-resistant strains also constitutes a serious worry to global health and livestock productivity. This study investigated Salmonella isolates from chicken and chicken meat products using the phenotypic antimicrobial screening as well as the molecular characteristics of Salmonella isolates. Upon serotyping of the isolates, the antimicrobial susceptibility profiling using a panel of 9 commonly used antimicrobials was done. Subsequently, the molecular profiles of all the isolates were further determined using Pulsed Field Gel Electrophoresis (PFGE) and the Whole Genome Multi-Locus Sequence Type (wgMLST) analysis in order to obtain the sequence types.
RESULTS
The PFGE data was input into FPQuest software, and the dendrogram generated was studied for possible genetic relatedness among the isolates. All the isolates were found to belong to the Salmonella Enteritidis serotype with notable resistance to tetracycline, gentamycin, streptomycin, and sulfadimidine. The S. Enteritidis isolates tested predominantly subtyped into the ST11 and ST1925, which was found to be a single cell variant of ST11. The STs were found to occur in chicken meats, foods, and live chicken cloacal swabs, which may indicate the persistence of the bacteria in multiple foci.
CONCLUSION
The data demonstrate the presence of S. Enteritidis among chickens, indicating its preference and reservoir status for enteric Salmonella pathogens.
背景
沙门氏菌是一种非常重要的食源性病原体，可导致人类患病。耐药菌株的出现也给全球健康和牲畜生产力带来严重担忧。本研究通过表型抗菌筛选以及沙门氏菌分离株的分子特征，对鸡肉和鸡肉制品中的沙门氏菌分离株进行了调查。对分离株进行血清分型后，使用一组9种常用抗菌药物进行了抗菌药敏分析。随后，为了获得序列类型，使用脉冲场凝胶电泳（PFGE）和全基因组多位点序列分型（wgMLST）分析进一步确定了所有分离株的分子图谱。
结果
将PFGE数据输入FPQuest软件，并研究生成的树形图以确定分离株之间可能的遗传相关性。所有分离株均属于肠炎沙门氏菌血清型，对四环素、庆大霉素、链霉素和磺胺二甲嘧啶具有显著抗性。测试的肠炎沙门氏菌分离株主要分为ST11和ST1925亚型，发现ST1925是ST11的单细胞变体。这些序列类型在鸡肉、食品和活鸡泄殖腔拭子中均有发现，这可能表明细菌在多个病灶中持续存在。
结论
数据表明鸡中存在肠炎沙门氏菌，表明其对肠道沙门氏菌病原体的偏好和储存宿主地位。
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