Instituto de Tecnologia Química e Biológica/Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal.
Biochem Biophys Res Commun. 2011 Jul 22;411(1):50-5. doi: 10.1016/j.bbrc.2011.06.084. Epub 2011 Jun 17.
The Escherichia coli bolA morphogene is very important in adaptation to stationary phase and stress response mechanisms. Genes of this family are widespread in gram negative bacteria and in eukaryotes. The expression of this gene is tightly regulated at transcriptional and post-transcriptional levels and its overexpression is known to induce round cellular morphology. The results presented in this report demonstrate that the H-NS protein, a pleiotropic regulator of gene expression, is a new transcriptional modulator of the bolA gene. In this work we show that and in vivo the levels of bolA are down-regulated by H-NS and in vitro this global regulator interacts directly with the bolA promoter region. Moreover, DNaseI foot-printing experiments mapped the interaction regions of H-NS and bolA and revealed that this global regulator binds not only one but both bolA promoters. We provide a new insight into the bolA regulation network demonstrating that H-NS represses the transcription of this important gene.
大肠杆菌 bolA 形态发生基因在适应静止期和应激反应机制方面非常重要。该家族的基因广泛存在于革兰氏阴性菌和真核生物中。该基因的表达在转录和转录后水平受到严格调控，其过表达已知会诱导细胞形态呈圆形。本报告中的结果表明，H-NS 蛋白是一种多效性基因表达调控因子，是 bolA 基因的新转录调节剂。在这项工作中，我们表明在体内 H-NS 下调 bolA 的水平，并且在体外，这种全局调节剂直接与 bolA 启动子区域相互作用。此外，DNaseI 足迹实验绘制了 H-NS 和 bolA 的相互作用区域，并揭示了该全局调节剂不仅结合一个 bolA 启动子，而且结合两个 bolA 启动子。我们提供了一个新的见解bolA 调控网络，表明 H-NS 抑制这个重要基因的转录。
