Department de Medicina Preventiva i Salut Pública, Facultat de Farmàcia, Universitat de València, Av. Vicent Andrés Estellés, 46100 Burjassot, Spain.
Food Chem Toxicol. 2011 Sep;49(9):2299-304. doi: 10.1016/j.fct.2011.06.030. Epub 2011 Jun 25.
A simple and rapid method effective for quantitative determination of deoxynivalenol (DON), T-2 toxin (T-2), HT-2 toxin (HT-2), zearalenone (ZEN), ochratoxin A (OTA), aflatoxins (AFs) B(1), B(2), G(1) and G(2) and fumonisins FB(1) and FB(2) in urine was developed. The urine was diluted with phosphate buffer solution (PBS) and thoroughly mixed. For clean-up and extraction, the mixture was loaded on a MYCO 6in1 IAC. Hybrid triple quadrupole-linear ion trap mass spectrometer (QTrap) was used for the detection. Extra tools for confirmation of selected mycotoxins in positive samples, Information Dependent Acquisition (IDA) experiments, were also developed. The use of immunoaffinity columns followed by the LC-MS/MS analysis showed acceptable average recoveries between 83% and 116% and reached acceptable precision values (relative standard deviation (RSD) ≤ 14%). In a pilot study with 27 volunteers, OTA, DON and AFG(2) were detected. However, this study needs to be extended in order to understand the relation between the mycotoxins intake and mycotoxin levels in human urine.
建立了一种用于尿液中脱氧雪腐镰刀菌烯醇（DON）、T-2 毒素（T-2）、HT-2 毒素（HT-2）、玉米赤霉烯酮（ZEN）、赭曲霉毒素 A（OTA）、黄曲霉毒素 B(1)、B(2)、G(1)和 G(2)、伏马菌素 FB(1)和 FB(2)定量测定的简单、快速方法。尿液用磷酸盐缓冲溶液（PBS）稀释并充分混合。为了进行净化和提取，将混合物加载到 MYCO 6in1 IAC 上。混合三重四极杆-线性离子阱质谱仪（QTrap）用于检测。还开发了用于确认阳性样品中选定真菌毒素的额外工具，即信息相关采集（IDA）实验。免疫亲和柱结合 LC-MS/MS 分析的使用显示出 83%至 116%之间可接受的平均回收率，并达到了可接受的精密度值（相对标准偏差（RSD）≤14%）。在 27 名志愿者的初步研究中，检测到了 OTA、DON 和 AFG(2)。然而，为了了解真菌毒素摄入与人体尿液中真菌毒素水平之间的关系，这项研究需要进一步扩展。
