Division of Pharmaceutical Biology, University of Basel, Switzerland.
Fitoterapia. 2011 Oct;82(7):1021-6. doi: 10.1016/j.fitote.2011.06.005. Epub 2011 Jun 25.
Lipophilic and polar extracts of iris rhizomes (Iris germanica) were submitted to a phytochemical profiling by a combination of HPLC-PDA-MS and semi-preparative HPLC/off-line microprobe NMR measurements. A total of 20 compounds were purified in sub-milligram to milligram amounts via two successive chromatographic steps. They were identified as isoflavones, isoflavone glycosides and acetovanillone by analysis of on-line MS and PDA, and off-line NMR data. A new isoflavone glycoside, iriflogenin-4'-O-gentiobioside, was subsequently isolated at preparative scale for full chemical characterization. This study demonstrates the applicability of the HPLC/off-line microprobe NMR approach as a robust means for rapid phytochemical profiling of plant extracts.
鸢尾根茎的亲脂性和极性提取物通过 HPLC-PDA-MS 和半制备 HPLC/离线微探头 NMR 测量的组合进行了植物化学特征分析。通过两个连续的色谱步骤，总共分离出了 20 种化合物，其含量达到亚毫克到毫克级。通过在线 MS 和 PDA 以及离线 NMR 数据分析，它们被鉴定为异黄酮、异黄酮糖苷和乙酰香草酮。随后，在制备规模上分离到一种新的异黄酮糖苷，鸢尾苷元-4'-O-龙胆二糖苷，以进行全面的化学表征。本研究证明了 HPLC/离线微探头 NMR 方法作为植物提取物快速植物化学特征分析的强大手段的适用性。
