Timmers Michael, Urban Sylvia
School of Applied Sciences, Health Innovations Research Institute, RMIT University, GPO Box 2476V Melbourne, Victoria 3001, Australia.
Nat Prod Commun. 2012 May;7(5):551-60.
On-line (HPLC-NMR) and off-line (HPLC, NMR and MS) methodologies were used to profile the constituents present in the crude extract of Lasiopetalum macrophyllum. On-flow and stop-flow HPLC-NMR supported the presence of trans-tiliroside and permitted partial identification of cis-tiliroside and 4'-methoxy-trans-tiliroside. Off-line isolation led to the unequivocal identification of four flavanoid glycosides including a new structural derivative, 4'-methoxycis-tiliroside. This is the first report of flavonoid glycosides occurring in this plant genus. In addition, a number of structure revisions have been proposed for previously reported flavonoid glycosides that were incorrectly assigned.
采用在线（HPLC-NMR）和离线（HPLC、NMR和MS）方法对大叶银叶树粗提物中的成分进行分析。流动注射和停流HPLC-NMR证实了反式椴树苷的存在，并对顺式椴树苷和4'-甲氧基反式椴树苷进行了部分鉴定。离线分离明确鉴定出四种黄酮苷，包括一种新的结构衍生物4'-甲氧基顺式椴树苷。这是该植物属中黄酮苷存在的首次报道。此外，还对先前报道的结构错误的黄酮苷提出了一些结构修正建议。
