Connor T H, Pier S M
University of Texas Health Science Center, School of Public Health, Houston 77225.
Mutat Res. 1990 Oct;245(2):129-33. doi: 10.1016/0165-7992(90)90012-9.
13 lead chromate-based pigments were assayed for mutagenicity and toxicity using Salmonella typhimurium TA100. The compounds were assayed with and without S9, both in the presence and absence of the chelating agent, nitrilotriacetic acid (NTA). In general, the use of NTA to solubilize the compounds resulted in mutagenicity and/or toxicity being observed where it had not in the absence of NTA, or being observed at lower concentrations than when water alone was used. Encapsulation of pigments with amorphous silica rendered these pigments non-mutagenic and non-toxic, indicating that the active moieties were biologically unavailable to the bacteria. Varying the percentage of silica encapsulation on one pigment, medium chrome yellow, indicated that 5% encapsulation did not alter the mutagenicity while 10% encapsulation inhibited the mutagenicity without or with NTA.
使用鼠伤寒沙门氏菌TA100对13种铬酸基颜料进行了致突变性和毒性检测。这些化合物在有和没有S9的情况下进行检测，检测时均同时存在和不存在螯合剂次氮基三乙酸（NTA）。一般来说，使用NTA溶解化合物会导致在不存在NTA时未观察到的致突变性和/或毒性，或者在比仅用水时更低的浓度下观察到致突变性和/或毒性。用无定形二氧化硅包裹颜料可使这些颜料无致突变性和无毒性，这表明活性部分对细菌无生物可利用性。改变一种颜料中二氧化硅包裹的百分比，即中铬黄，结果表明5%的包裹率不会改变致突变性，而10%的包裹率在有无NTA的情况下均会抑制致突变性。
