rk39 免疫层析试验检测尿液诊断内脏利什曼病的评价。
Evaluation of rk39 immunochromatographic test with urine for diagnosis of visceral leishmaniasis.
机构信息
Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221 005, U.P., India.
出版信息
Trans R Soc Trop Med Hyg. 2011 Sep;105(9):537-9. doi: 10.1016/j.trstmh.2011.05.008. Epub 2011 Jun 25.
Abstract
This study evaluates commercially available rK39 immunochromatographic strips using urine for diagnosis of visceral leishmaniasis (VL). Freshly collected urine and serum samples of 280 parasitologically confirmed VL patients and 66 endemic healthy controls (EHC), 48 nonendemic healthy controls (NEHC) and 45 different diseases were tested with rK39 strips. The sensitivity of rK39 in urine was 96.4% while the specificity was low varying from 66.7% in EHC, 77.08% in NEHC to 62.2% in different diseases. With serum, sensitivity was 100% whereas the specificity was 100%, 92.4% and 95.55% for the respective control groups. In the present format, the immunochromatographic strips cannot be used for the diagnosis of VL using urine samples.
摘要
本研究评估了市售 rK39 免疫层析条，采用尿液样本用于内脏利什曼病（VL）的诊断。对 280 例经寄生虫学证实的 VL 患者和 66 例地方性健康对照（EHC）、48 例非地方性健康对照（NEHC）和 45 种不同疾病的新鲜采集的尿液和血清样本进行 rK39 条检测。rK39 在尿液中的灵敏度为 96.4%，特异性较低，在 EHC 中为 66.7%，在 NEHC 中为 77.08%，在不同疾病中为 62.2%。使用血清时，相应对照组的灵敏度均为 100%，特异性分别为 100%、92.4%和 95.55%。按照目前的形式，免疫层析条不能用于尿液样本中 VL 的诊断。
相似文献
1
Evaluation of rk39 immunochromatographic test with urine for diagnosis of visceral leishmaniasis.rk39 免疫层析试验检测尿液诊断内脏利什曼病的评价。
Trans R Soc Trop Med Hyg. 2011 Sep;105(9):537-9. doi: 10.1016/j.trstmh.2011.05.008. Epub 2011 Jun 25.
2
Is urine a reliable clinical sample for the diagnosis of human visceral leishmaniasis? A systematic review and meta-analysis.尿液是诊断人类内脏利什曼病的可靠临床样本吗？一项系统评价与荟萃分析。
Parasitol Int. 2018 Oct;67(5):575-583. doi: 10.1016/j.parint.2018.05.008. Epub 2018 May 19.
3
Testing urine samples with rK39 strip as the simplest non-invasive field diagnosis for visceral leishmaniasis: an early report from eastern India.使用rK39试纸条检测尿液样本作为内脏利什曼病最简单的非侵入性现场诊断方法：来自印度东部的早期报告。
J Postgrad Med. 2012 Jul-Sep;58(3):180-4. doi: 10.4103/0022-3859.101378.
4
Serological diagnosis of Indian visceral leishmaniasis: direct agglutination test versus rK39 strip test.印度内脏利什曼病的血清学诊断：直接凝集试验与rK39试纸条检测对比
Trans R Soc Trop Med Hyg. 2006 Jun;100(6):533-7. doi: 10.1016/j.trstmh.2005.08.018. Epub 2005 Dec 2.
5
Performance of latex agglutination test (KAtex) in diagnosis of visceral leishmaniasis in Iran.乳胶凝集试验（KAtex）在伊朗内脏利什曼病诊断中的表现。
Iran J Immunol. 2009 Dec;6(4):202-7.
6
Evaluation of rK28 antigen for serodiagnosis of visceral Leishmaniasis in India.评估 rK28 抗原在印度内脏利什曼病血清学诊断中的应用。
Clin Microbiol Infect. 2012 Jan;18(1):81-5. doi: 10.1111/j.1469-0691.2011.03540.x. Epub 2011 Jul 1.
7
Evaluation of a new recombinant K39 rapid diagnostic test for Sudanese visceral leishmaniasis.一种用于苏丹内脏利什曼病的新型重组K39快速诊断测试的评估。
Am J Trop Med Hyg. 2006 Jan;74(1):76-80.
8
Performance of rK39-based immunochromatographic rapid diagnostic test for serodiagnosis of visceral leishmaniasis using whole blood, serum and oral fluid.rK39 免疫层析快速诊断试验检测全血、血清和唾液用于内脏利什曼病血清学诊断的性能评估。
PLoS One. 2020 Apr 2;15(4):e0230610. doi: 10.1371/journal.pone.0230610. eCollection 2020.
9
Heterogeneity of Leishmania donovani parasites complicates diagnosis of visceral leishmaniasis: comparison of different serological tests in three endemic regions.杜氏利什曼原虫寄生虫的异质性使内脏利什曼病的诊断复杂化：三个流行地区不同血清学检测的比较
PLoS One. 2015 Mar 3;10(3):e0116408. doi: 10.1371/journal.pone.0116408. eCollection 2015.
10
Validation of rK39 immunochromatographic test and direct agglutination test for the diagnosis of Mediterranean visceral leishmaniasis in Spain.rK39 免疫层析试验和直接凝集试验对西班牙地中海内脏利什曼病的诊断验证。
PLoS Negl Trop Dis. 2018 Mar 1;12(3):e0006277. doi: 10.1371/journal.pntd.0006277. eCollection 2018 Mar.
引用本文的文献
1
Serology for visceral leishmaniasis: How trusty is the accuracy reported by the manufacturers?内脏利什曼病血清学检测：制造商报告的准确性有多可靠？
Rev Soc Bras Med Trop. 2023 Feb 20;56:e0358. doi: 10.1590/0037-8682-0358-2022. eCollection 2023.
2
Evaluation of urine sample for diagnosis of visceral leishmaniasis using rK-39 immunochromatographic test in Northwest Ethiopia.利用 rK-39 免疫层析试验评估埃塞俄比亚西北部尿液样本用于内脏利什曼病的诊断。
PLoS One. 2022 Feb 7;17(2):e0263696. doi: 10.1371/journal.pone.0263696. eCollection 2022.
3
Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis.非侵入性生物样本用于检测和诊断原生动物寄生虫引起的感染：系统评价和荟萃分析。
Int J Mol Sci. 2020 Feb 29;21(5):1684. doi: 10.3390/ijms21051684.
4
Noninvasive Diagnosis of Visceral Leishmaniasis: Development and Evaluation of Two Urine-Based Immunoassays for Detection of Leishmania donovani Infection in India.内脏利什曼病的非侵入性诊断：两种基于尿液的免疫测定法在印度检测杜氏利什曼原虫感染的开发与评估
PLoS Negl Trop Dis. 2016 Oct 14;10(10):e0005035. doi: 10.1371/journal.pntd.0005035. eCollection 2016 Oct.
5
Evaluation of diagnostic performance of rK28 ELISA using urine for diagnosis of visceral leishmaniasis.评估使用尿液的rK28酶联免疫吸附测定法对内脏利什曼病的诊断性能。
Parasit Vectors. 2016 Jul 4;9(1):383. doi: 10.1186/s13071-016-1667-2.
6
Developments in Diagnosis of Visceral Leishmaniasis in the Elimination Era.消除时代内脏利什曼病的诊断进展
J Parasitol Res. 2015;2015:239469. doi: 10.1155/2015/239469. Epub 2015 Dec 30.
7
Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic region of India.应用 rK-39 条带试验检测尿液诊断印度内脏利什曼病流行区病例。
Am J Trop Med Hyg. 2013 Feb;88(2):222-6. doi: 10.4269/ajtmh.2012.12-0489. Epub 2012 Nov 13.
8
Parasitic infections in HIV infected individuals: diagnostic & therapeutic challenges.HIV 感染者的寄生虫感染：诊断和治疗挑战。
Indian J Med Res. 2011 Dec;134(6):878-97. doi: 10.4103/0971-5916.92633.
本文引用的文献
1
Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh.在孟加拉国一个流行地区使用尿液进行 rK-39 试纸条检测诊断内脏利什曼病的评估。
Parasit Vectors. 2010 Nov 26;3:114. doi: 10.1186/1756-3305-3-114.
2
3
Comparative evaluation of parasitology and serological tests in the diagnosis of visceral leishmaniasis in India: a phase III diagnostic accuracy study.印度内脏利什曼病诊断中寄生虫学和血清学检测的比较评估：一项III期诊断准确性研究。
Trop Med Int Health. 2007 Feb;12(2):284-9. doi: 10.1111/j.1365-3156.2006.01775.x.
4
Cystatin C as a marker of immune complex-associated renal impairment in a Sudanese population with visceral leishmaniasis.胱抑素C作为苏丹内脏利什曼病患者免疫复合物相关肾损伤的标志物。
Am J Trop Med Hyg. 2006 Nov;75(5):864-8.
5
Elimination of leishmaniasis (kala-azar) from the Indian subcontinent is technically feasible & operationally achievable.在印度次大陆消除利什曼病（黑热病）在技术上是可行的，在操作上也是可以实现的。
Indian J Med Res. 2006 Mar;123(3):195-6.
6
Rapid, noninvasive diagnosis of visceral leishmaniasis in India: comparison of two immunochromatographic strip tests for detection of anti-K39 antibody.印度内脏利什曼病的快速、非侵入性诊断：两种检测抗K39抗体的免疫层析试纸条试验的比较
J Clin Microbiol. 2006 Jan;44(1):251-3. doi: 10.1128/JCM.44.1.251-253.2006.
7
Serological diagnosis of Indian visceral leishmaniasis: direct agglutination test versus rK39 strip test.印度内脏利什曼病的血清学诊断：直接凝集试验与rK39试纸条检测对比
Trans R Soc Trop Med Hyg. 2006 Jun;100(6):533-7. doi: 10.1016/j.trstmh.2005.08.018. Epub 2005 Dec 2.
8
Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis.用于内脏利什曼病诊断的尿液样本直接凝集试验。
Am J Trop Med Hyg. 2004 Jan;70(1):78-82.
9
Detection of anti-Leishmania immunoglobulin G antibodies in urine specimens of dogs with leishmaniasis.利什曼病犬尿液标本中抗利什曼原虫免疫球蛋白G抗体的检测
Clin Diagn Lab Immunol. 2003 Sep;10(5):849-55. doi: 10.1128/cdli.10.5.849-855.2003.
10
Enzyme-linked immunosorbent assay for recombinant K39 antigen in diagnosis and prognosis of Indian visceral leishmaniasis.用于印度内脏利什曼病诊断和预后的重组K39抗原酶联免疫吸附测定法。
Clin Diagn Lab Immunol. 2001 Nov;8(6):1220-4. doi: 10.1128/CDLI.8.6.1220-1224.2001.
Zotero 插件