Department of Food Science and Technology, Aristotle University, Box 250, 54124 Thessaloniki, Greece.
Bioresour Technol. 2011 Sep;102(17):8159-64. doi: 10.1016/j.biortech.2011.06.027. Epub 2011 Jun 12.
The adaptive response of the fungus Blakeslea trispora to the oxidative stress induced by elevated dissolved oxygen concentrations during carotene production was investigated by measuring the specific activities of catalase (CAT) and superoxide dismutase (SOD) and the micromorphology of the fungus using a computerized image analysis system. Changes in the ratio of the volume of air (V(a)) over the medium and the volume of medium (V(m)) in the flask caused changes of the morphology of microorganism from clumps to pellets and increases in the specific activities of CAT and SOD. The oxidative stress in B. trispora resulted in a significant increase in carotene production, and a maximum proportion of β-carotene (60%), γ-carotene (50%), and lycopene (10%) (as percentages of total carotenes) was observed at a ratio V(a)/V(m) of 15.7, 4.0 and 1.5, respectively. The highest concentration of carotenes (115.0mg/g dry biomass) was obtained in V(a)/V(m) ratio of 9.0.
采用比色法和计算机图像分析系统研究了粘性红酵母（Blakeslea trispora）在生产类胡萝卜素过程中，为适应因溶氧升高所导致的氧化胁迫，其胞内过氧化氢酶（CAT）和超氧化物歧化酶（SOD）比活力以及细胞微观形态的变化。摇瓶中通气比（V(a)/V(m)）的变化会引起微生物形态由聚集体向颗粒体的转变，并导致 CAT 和 SOD 比活力的增加。粘性红酵母氧化胁迫会导致类胡萝卜素产量的显著增加，当通气比分别为 15.7、4.0 和 1.5 时，β-胡萝卜素（60%）、γ-胡萝卜素（50%）和番茄红素（10%）（分别占总类胡萝卜素的比例）的比例达到最大。当通气比为 9.0 时，粘性红酵母的类胡萝卜素浓度最高（115.0mg/g 干生物质）。
