Azizi Maryam, Zare Davood, Sepahi Abbas Akhavan, Azin Mehrdad
Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.
Folia Microbiol (Praha). 2024 Oct 3. doi: 10.1007/s12223-024-01202-y.
Among carotenoids, ꞵ-carotene has the highest biological activity and is found as an all-trans isomer in many biological systems. Blakeslea trispora is a microorganism that is of interest to industries for the commercial production of ꞵ-carotene. This study investigated the effect of different bacteria on carotenogenesis in B. trispora. The B. trispora bisexual mold was cultured in a production medium, and different bacterial cells were added to it after 24 h. Then, the culture conditions and the culture medium were optimized in the presence of the selected bacteria using the experimental design. The percentage of carotenoids obtained from the mixed culture was determined using high-performance liquid chromatography (HPLC). Results showed that Kocuria rhizophila had the greatest effect on increasing the production of carotenoids in B. trispora. The highest content of carotenoids obtained during optimization was 770 ± 7.5 mg/L, a 6.8-fold increase compared to the control. HPLC analysis of carotenoids indicated the presence of two main peaks, ꞵ-carotene and -carotene, in which the primary carotenoid was ꞵ-carotene followed by -carotene with a lower content. Therefore, due to the importance of ꞵ-carotene in industry, the use of biostimulants is one of the appropriate strategies to increase the production of this pigment in industry.
在类胡萝卜素中，β-胡萝卜素具有最高的生物活性，并且在许多生物系统中以全反式异构体的形式存在。三孢布拉氏霉菌是一种微生物，因其可用于商业生产β-胡萝卜素而受到工业界的关注。本研究调查了不同细菌对三孢布拉氏霉菌类胡萝卜素生成的影响。将三孢布拉氏霉菌的两性霉菌接种于生产培养基中，24小时后添加不同的细菌细胞。然后，使用实验设计在所选细菌存在的情况下优化培养条件和培养基。使用高效液相色谱法（HPLC）测定混合培养物中获得的类胡萝卜素百分比。结果表明，嗜根考克氏菌对提高三孢布拉氏霉菌类胡萝卜素产量的影响最大。优化过程中获得的类胡萝卜素最高含量为770±7.5mg/L，与对照相比增加了6.8倍。类胡萝卜素的HPLC分析表明存在两个主要峰，β-胡萝卜素和γ-胡萝卜素，其中主要类胡萝卜素是β-胡萝卜素，其次是含量较低的γ-胡萝卜素。因此，由于β-胡萝卜素在工业中的重要性，使用生物刺激剂是提高该色素在工业中产量的合适策略之一。
