Biology Program, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Bioresour Technol. 2011 Sep;102(17):8323-6. doi: 10.1016/j.biortech.2011.06.021. Epub 2011 Jun 12.
Several new observations related to the enhancement effect of Tween 80 on the mycelial growth and exopolysaccharide production by the submerged fermentation of Pleurotus tuber-regium were reported in the present study. Firstly, it was found that the addition of Tween 80 on the 5th day could significantly increase the glucose consumption rate at the later stage of the fermentation compared to the control. Secondly, addition of Tween 80 could maintain the intact structure of the mycelial pellets of P. tuber-regium with little signs of disintegration as observed under microscope and kept the pH value of the fermentation broth at an acidic level lower than that of the control. Thirdly, the oleic acid (C18:1) composition in the mycelial cell membrane was significantly increased from 2.6% (in the control) to 18.5% (with addition of Tween 80) coincided with a decrease in the concentration of Tween 80 in the culture medium. These new findings provide some important insight to the elucidation of the detailed mechanism by which Tween 80 is used as a stimulatory agent in the submerged fermentation of mushroom mycelium.
本研究报道了吐温 80 对蛹虫草深层发酵中菌丝生长和胞外多糖生产的促进作用的一些新观察结果。首先，发现与对照组相比，第 5 天添加吐温 80 可以显著提高发酵后期的葡萄糖消耗率。其次，添加吐温 80 可以保持蛹虫草菌丝球的完整结构，显微镜下几乎没有解体的迹象，并使发酵液的 pH 值保持在低于对照组的酸性水平。第三，菌丝细胞膜中的油酸（C18:1）组成从 2.6%（对照组）显著增加到 18.5%（添加吐温 80），同时培养基中吐温 80 的浓度降低。这些新发现为阐明吐温 80 作为蘑菇菌丝体深层发酵中刺激剂的详细机制提供了一些重要的见解。
