Department of Chemical Engineering, Tsinghua University, Beijing, People's Republic of China.
Appl Biochem Biotechnol. 2010 Jan;160(2):621-31. doi: 10.1007/s12010-008-8507-6. Epub 2009 Jan 21.
This paper is concerned with the optimization of effect factors for mycelial growth and exopolysaccharide production by Schizophyllum commune by one-factor-at-a-time and orthogonal methods. The one-factor-at-a-time method was adopted to investigate the effects of six different compounds (sodium carboxymethylcellulose, L: -glutamic acid, V(B1), naphthalene acetic acid, oleic acid, and Tween 80) on mycelial growth and exopolysaccharide production. Among these factors, oleic acid, V(B1) and Tween 80 were identified to be the most important factors. Subsequently, the concentration of oleic acid, V(B1) and Tween 80 were optimized using the orthogonal matrix method. The effects of the factors on the mycelial growth of S. commune were in the order of oleic acid > V(B1) > Tween 80, and those on exopolysaccharide production were in the same order. The optimal concentration for mycelia and exopolysaccharide were determined as oleic acid 0.1% (v/v), V(B1) 0.5 mg/L, and Tween 80 6 mg/L. The subsequent verification experiments confirmed the validity of the models. Under this optimized conditions in shake flask culture, the mycelial yield and exo-biopolymer production were 25.93 and 2.79 g/L, respectively, which were considerably higher than those obtained in the preliminary studies. The result was further confirmed in a 7-L fermentor experiments.
本文采用单因素法和正交试验法优化了裂褶菌菌丝体生长和胞外多糖生产的影响因素。采用单因素法研究了 6 种不同化合物（羧甲基纤维素钠、L：-谷氨酸、VB1、萘乙酸、油酸、吐温 80）对裂褶菌菌丝体生长和胞外多糖生产的影响。其中，油酸、VB1 和吐温 80 被确定为最重要的因素。然后，采用正交矩阵法优化了油酸、VB1 和吐温 80 的浓度。这些因素对裂褶菌菌丝体生长的影响顺序为油酸>VB1>吐温 80，对胞外多糖生产的影响顺序相同。确定菌丝体和胞外多糖的最佳浓度分别为油酸 0.1%（v/v）、VB1 0.5mg/L 和吐温 80 6mg/L。随后的验证实验证实了模型的有效性。在摇瓶培养的优化条件下，菌丝体产量和胞外生物聚合物产量分别为 25.93 和 2.79g/L，明显高于初步研究中的产量。在 7L 发酵罐实验中进一步证实了这一结果。
