Grupo de Micobacterias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal.
In Vivo. 2011 Jul-Aug;25(4):597-602.
To determine whether tetracycline competes with ethidium bromide (EB), used as substrate of efflux pumps, for extrusion by the efflux pump system of Salmonella enteritidis NCTC 13349 reference strain.
Evaluation of efflux of EB in competition with tetracycline was conducted with the aid of an automated EB method and by EB agar method.
The EB agar method demonstrated that the accumulation of EB by S. enteritidis NCTC 13349 reference strain is reduced by high concentrations of tetracycline and that tetracycline accumulates within the cells. The use of the automated EB method confirmed the EB agar results and demonstrated that accumulation of EB is not affected by tetracycline unless the concentration of the antibiotic exceeds 100 mg/l.
These results suggest that tetracycline is preferentially retained and that EB is preferentially extruded by the efflux pump of Sientintidis NCTC 13349.
确定四环素是否与用作外排泵底物的溴化乙锭（EB）竞争，由肠炎沙门氏菌 NCTC 13349 参考菌株的外排泵系统排出。
借助自动 EB 方法和 EB 琼脂方法评估四环素与 EB 的外排竞争。
EB 琼脂法表明，高浓度四环素可降低肠炎沙门氏菌 NCTC 13349 参考菌株对 EB 的积累，并且四环素在细胞内积累。自动 EB 方法证实了 EB 琼脂结果，并表明只有当抗生素浓度超过 100mg/L 时，EB 的积累才不受四环素的影响。
这些结果表明，四环素优先保留，而 EB 优先由肠炎沙门氏菌 NCTC 13349 的外排泵排出。
