葡萄提取物对氧化应激调节相关酶体外活性的影响。
Effects of grape extracts on the in vitro activity of enzymes involved in oxidative stress regulation.
机构信息
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, 41221, Larissa, Greece.
出版信息
In Vivo. 2011 Jul-Aug;25(4):657-62.
BACKGROUND/AIM: A number of in vitro and in vivo studies have investigated the potential preventive activity of grape extracts against different diseases, and have mostly focused on their antioxidant properties. The present study examined the effects of 21 extracts from stem, skin and berry from Greek grape varieties on the activity of enzymes involved in regulation of oxidative stress, namely xanthine oxidase (XO), catalase (CAT) and superoxide dismutase (SOD).
MATERIALS AND METHODS
The effects of the extracts on the enzymatic activity of XO, CAT and SOD were studied spectrophotometrically.
RESULTS
The tested extracts inhibited CAT and XO activity, while higher extract concentrations are required to induce SOD. However, stem and skin extracts exhibited a different inhibitory pattern against CAT and XO compared to berry extracts. The observed differences are possibly attributed to the extract polyphenolic composition.
CONCLUSION
Although the induction of SOD activity suggests an antioxidant capacity, the inhibition of CAT and XO indicates a pro-oxidant action. In general, the extracts showed pro-oxidant activity, possibly dependent on both their polyphenolic composition and concentration.
背景/目的：许多体外和体内研究已经研究了葡萄提取物对不同疾病的潜在预防活性，并且主要集中在其抗氧化特性上。本研究检查了来自希腊葡萄品种的茎，皮和浆果的 21 种提取物对参与氧化应激调节的酶（即黄嘌呤氧化酶（XO），过氧化氢酶（CAT）和超氧化物歧化酶（SOD））的活性的影响。
材料和方法
使用分光光度法研究了提取物对 XO，CAT 和 SOD 的酶活性的影响。
结果
测试的提取物抑制 CAT 和 XO 活性，而诱导 SOD 需要更高的提取物浓度。然而，与浆果提取物相比，茎和皮提取物对 CAT 和 XO 的抑制模式不同。观察到的差异可能归因于提取物的多酚组成。
结论
尽管 SOD 活性的诱导表明抗氧化能力，但 CAT 和 XO 的抑制表明其具有促氧化剂作用。一般来说，提取物表现出促氧化剂活性，这可能取决于其多酚组成和浓度。
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