Department of Bioengineering, Clemson University, 301 Rhodes Hall, Clemson, SC 29634-0905, USA.
Antimicrob Agents Chemother. 2011 Sep;55(9):4379-85. doi: 10.1128/AAC.01056-10. Epub 2011 Jun 27.
Bacterial infections by antibiotic-resistant Staphylococcus aureus strains are among the most common postoperative complications in surgical hernia repair with synthetic mesh. Surface coating of medical devices/implants using antibacterial peptides and enzymes has recently emerged as a potentially effective method for preventing infections. The objective of this study was to evaluate the in vitro antimicrobial activity of hernia repair meshes coated by the antimicrobial enzyme lysostaphin at different initial concentrations. Lysostaphin was adsorbed on pieces of polypropylene (Ultrapro) mesh with binding yields of ∼10 to 40% at different coating concentrations of between 10 and 500 μg/ml. Leaching of enzyme from the surface of all the samples was studied in 2% (wt/vol) bovine serum albumin in phosphate-buffered saline buffer at 37°C, and it was found that less than 3% of adsorbed enzyme desorbed from the surface after 24 h of incubation. Studies of antibacterial activity against a cell suspension of S. aureus were performed using turbidity assay and demonstrated that the small amount of enzyme leaching from the mesh surface contributes to the lytic activity of the lysostaphin-coated samples. Colony counting data from the broth count (model for bacteria in wound fluid) and wash count (model for colonized bacteria) for the enzyme-coated samples showed significantly decreased numbers of CFU compared to uncoated samples (P < 0.05). A pilot in vivo study showed a dose-dependent efficacy of lysostaphin-coated meshes in a rat model of S. aureus infection. The antimicrobial activity of the lysostaphin-coated meshes suggests that such enzyme-leaching surfaces could be efficient at actively resisting initial bacterial adhesion and preventing subsequent colonization of hernia repair meshes.
耐抗生素金黄色葡萄球菌引起的细菌感染是外科疝修补术用合成网片后最常见的并发症之一。最近，使用抗菌肽和酶对医疗器械/植入物进行表面涂层已成为一种预防感染的有效方法。本研究的目的是评估不同初始浓度的抗菌酶溶葡萄球菌素涂层疝修补网片的体外抗菌活性。溶葡萄球菌素以约 10%至 40%的结合率被吸附在不同浓度为 10 至 500μg/ml 的聚丙稀（Ultrapro）网片上。在 37°C 的 2%（重量/体积）牛血清白蛋白磷酸盐缓冲液中研究了所有样品表面酶的浸出情况，结果发现孵育 24 小时后，仅有不到 3%的吸附酶从表面解吸。使用浊度测定法对金黄色葡萄球菌细胞悬浮液进行了抗菌活性研究，结果表明，从小网片表面浸出的少量酶有助于溶葡萄球菌素涂层样品的溶菌活性。来自肉汤计数（伤口液中细菌模型）和洗出计数（定植细菌模型）的酶涂层样品的细菌计数数据表明，与未涂层样品相比，CFU 数量显著减少（P < 0.05）。一项初步的体内研究表明，在金黄色葡萄球菌感染的大鼠模型中，溶葡萄球菌素涂层网片具有剂量依赖性的疗效。溶葡萄球菌素涂层网片的抗菌活性表明，这种酶浸出表面可以有效抵抗初始细菌黏附，并防止随后疝修补网片的定植。
