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所产生的重组溶葡萄球菌抗菌肽在小鼠模型中治疗耐甲氧西林金黄色葡萄球菌（MRSA）皮肤感染的效率。

efficiency of the produced recombinant lysostaphin antimicrobial peptide in treatment of methicillin-resistant (MRSA) skin infection in a mouse model.

作者信息

Sadoogh Abbasian Shabnam, Ghaznavi-Rad Ehsanollah, Sadelaji Samira, Abtahi Hamid

机构信息

Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.

Department of Medical Microbiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.

出版信息

Iran J Microbiol. 2023 Apr;15(2):243-250. doi: 10.18502/ijm.v15i2.12476.

DOI:10.18502/ijm.v15i2.12476
PMID:37193239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10183077/
Abstract

BACKGROUND AND OBJECTIVES

secretes an antimicrobial compound called lysostaphin, which has bactericidal properties. It destroys staphylococci through the hydrolysis of peptidoglycan in the cell wall. Therefore, this unique property indicates the high ability of lysostaphin in the treatment of staphylococcal infections and is considered as an anti-staphylococcal agent.

MATERIALS AND METHODS

BL21 (DE3) competent cells were transformed with pET32a-lysostaphin clone and induced by isopropyl-β-D-thio-galactoside (IPTG). The recombinant protein was purified by affinity chromatography. Recombinant lysostaphin -A-based ointment was used for external wound healing in animal model. activity of ointment was evaluated by clinical evidences and cytological microscopic assessment.

RESULTS

Our results showed the recombinant protein was produced exactly. The results of checkerboard tests showed MIC, MBC and antibacterial activity test an acute reduction of cell viability during the use of lysostaphin, and SEM results approved the intense wrecking effects of lysostaphin in combination on bacterial cells. Macroscopic findings and microscopic data showed that the recombinant lysostaphin ointment was effective on excisional wound healing.

CONCLUSION

Our findings proved that the recombinant lysostaphin ointment was effective on wound healing due to infection.

摘要

背景与目的

[某种物质]分泌一种名为溶葡萄球菌酶的抗菌化合物，其具有杀菌特性。它通过水解细胞壁中的肽聚糖来破坏葡萄球菌。因此，这种独特特性表明溶葡萄球菌酶在治疗葡萄球菌感染方面具有很高的能力，被视为一种抗葡萄球菌剂。

材料与方法

用pET32a - 溶葡萄球菌酶克隆转化BL21(DE3)感受态细胞，并用异丙基 - β - D - 硫代半乳糖苷（IPTG）诱导。重组蛋白通过亲和层析纯化。基于重组溶葡萄球菌酶 - A的软膏用于动物模型的外部伤口愈合。通过临床证据和细胞学显微镜评估来评价软膏的活性。

结果

我们的结果表明重组蛋白精确产生。棋盘试验结果显示最低抑菌浓度（MIC）、最低杀菌浓度（MBC）以及抗菌活性试验表明在使用溶葡萄球菌酶期间细胞活力急剧下降，扫描电子显微镜（SEM）结果证实溶葡萄球菌酶联合使用对细菌细胞具有强烈的破坏作用。宏观观察结果和微观数据表明重组溶葡萄球菌酶软膏对切除伤口愈合有效。

结论

我们的研究结果证明重组溶葡萄球菌酶软膏对因[感染类型未明确]感染导致的伤口愈合有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/a8572eb76432/IJM-15-243-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/3afdc358b808/IJM-15-243-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/2167cf3aa13f/IJM-15-243-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/8c2ffeb2c878/IJM-15-243-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/d2d31ab541f3/IJM-15-243-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/7d10603df0a4/IJM-15-243-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/2b7243086c37/IJM-15-243-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/d8b8093eea23/IJM-15-243-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/a8572eb76432/IJM-15-243-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/3afdc358b808/IJM-15-243-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/2167cf3aa13f/IJM-15-243-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/8c2ffeb2c878/IJM-15-243-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/d2d31ab541f3/IJM-15-243-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/7d10603df0a4/IJM-15-243-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/2b7243086c37/IJM-15-243-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/d8b8093eea23/IJM-15-243-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ffc/10183077/a8572eb76432/IJM-15-243-g008.jpg

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本文引用的文献

1
Investigation of Morchella esculenta and Morchella conica for their antibacterial potential against methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pyogenes.调查羊肚菌和柱状羊肚菌的抗菌潜力，针对耐甲氧西林金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和化脓性链球菌。
Arch Microbiol. 2022 Jun 14;204(7):391. doi: 10.1007/s00203-022-03003-8.
2
Peptidoglycan: Structure, Synthesis, and Regulation.肽聚糖：结构、合成与调控。
EcoSal Plus. 2021 Jan;9(2). doi: 10.1128/ecosalplus.ESP-0010-2020.
3
Therapeutic applications of lysostaphin against Staphylococcus aureus.
溶葡萄球菌酶治疗金黄色葡萄球菌感染的应用。
J Appl Microbiol. 2021 Sep;131(3):1072-1082. doi: 10.1111/jam.14985. Epub 2021 Jan 18.
4
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology.耐甲氧西林金黄色葡萄球菌：分子特征、进化与流行病学。
Clin Microbiol Rev. 2018 Sep 12;31(4). doi: 10.1128/CMR.00020-18. Print 2018 Oct.
5
Production of Recombinant Antimicrobial Polymeric Protein Beta Casein-E 50-52 and Its Antimicrobial Synergistic Effects Assessment with Thymol.重组抗菌聚合蛋白β-酪蛋白-E 50-52的制备及其与百里香酚的抗菌协同效应评估
Molecules. 2017 May 31;22(6):822. doi: 10.3390/molecules22060822.
6
Lysostaphin: A Staphylococcal Bacteriolysin with Potential Clinical Applications.溶葡萄球菌酶：一种具有潜在临床应用价值的葡萄球菌溶菌素
Pharmaceuticals (Basel). 2010 Apr 19;3(4):1139-1161. doi: 10.3390/ph3041139.
7
Inhibition of methicillin-resistant Staphylococcus aureus (MRSA) by antimicrobial peptides (AMPs) and plant essential oils.抗菌肽和植物精油对耐甲氧西林金黄色葡萄球菌（MRSA）的抑制作用
Pharm Biol. 2016 Dec;54(12):3136-3150. doi: 10.1080/13880209.2016.1190763. Epub 2016 Jun 1.
8
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Jundishapur J Microbiol. 2014 May;7(5):e10009. doi: 10.5812/jjm.10009. Epub 2014 May 1.
9
Skin and soft tissue infections.皮肤和软组织感染。
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10
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Biotechnol Adv. 2013 Sep-Oct;31(5):688-705. doi: 10.1016/j.biotechadv.2012.10.003. Epub 2012 Nov 9.