Chalk River Laboratories, Atomic Energy of Canada Limited, Chalk River, ON, Canada K0J 1J0.
Health Phys. 2011 Aug;101(2):148-53. doi: 10.1097/HP.0b013e318213b9e6.
Following a radiological or nuclear emergency involving uranium exposure, rapid analytical methods are needed to analyze the concentration of uranium isotopes in human urine samples for early dose assessment. The inductively coupled plasma mass spectrometry (ICP-MS) technique, with its high sample throughput and high sensitivity, has advantages over alpha spectrometry for uranium urinalysis after minimum sample preparation. In this work, a rapid sample preparation method using an anion exchange chromatographic column was developed to separate uranium from the urine matrix. A high-resolution sector field ICP-MS instrument, coupled with a high sensitivity desolvation sample introduction inlet, was used to determine uranium isotopes in the samples. The method can analyze up to 24 urine samples in two hours with the limits of detection of 0.0014, 0.10, and 2.0 pg mL(-1) for (234)U, (235)U, and (238)U, respectively, which meet the requirement for isotopic analysis of uranium in a radiation emergency.
在涉及铀暴露的放射性或核紧急情况下，需要快速分析方法来分析人尿液样本中铀同位素的浓度，以便进行早期剂量评估。与α谱法相比，电感耦合等离子体质谱（ICP-MS）技术在经过最小样品制备后，对铀尿液分析具有高通量和高灵敏度的优势。在这项工作中，开发了一种使用阴离子交换色谱柱的快速样品制备方法，以将铀从尿液基质中分离出来。高分辨率扇形场 ICP-MS 仪器与高灵敏度解吸进样入口耦合，用于测定样品中的铀同位素。该方法可以在两个小时内分析多达 24 个尿液样本，对于 (234)U、(235)U 和 (238)U 的检测限分别为 0.0014、0.10 和 2.0 pg mL(-1)，满足辐射紧急情况下铀同位素分析的要求。
