Kawaura Akihiko, Tanida Noritoshi, Akiyama Junichi, Nonaka Kouji, Mizutani Masatoshi, Sawada Kenji, Nakagawa Kimie, Tsugawa Naoko, Izumi Keisuke, Ii Kunio, Okano Toshio, Takeda Eiji
Department of Physical Therapy, School of Health Care and Social Welfare, KIBI International University, Takahashi, Okayama, Japan.
Acta Med Okayama. 2011 Jun;65(3):193-7. doi: 10.18926/AMO/46631.
Sixty-three male 5-week-old Syrian hamsters received the carcinogen N-nitrosobis(2-oxopropyl)amine (BOP) s.c. in 5 weekly injections (the first, 70 mg/kg body, and the remaining, 20mg/kg each). The hamsters that received BOP were given intragastric administration of 0.2 ml of medium chain triglyceride (MCT) with or without 0.04 μg of 1α-hydroxyvitamin D3 [1α(OH)D3] through a feeding tube for 12 weeks. Thus, 3 groups were assigned:Group 1;BOP alone (n＝20), Group 2;BOP＋MCT (n＝18) and Group 3;BOP＋1α(OH)D3 (n＝25). The mean body weight of Group 3 was lower than those of Groups 1 and 2 at the end of the experiment (p＜0.001,Tukey-Kramer HSD test). At the end of week 12, all surviving hamsters were put to sleep. The incidences of liver tumors were 80%, 72% and 32% in Groups 1, 2 and 3, respectively. The incidence of tumors in Group 3 was significantly lower than in Group 1 and Group 2 (p＜0.05, χ2-test). All tumors were cholangiocarcinoma. These results indicated that BOP-induced cholangiocarcinogenesis was suppressed by the supplemental administration of 1α(OH)D3.
63只5周龄的雄性叙利亚仓鼠，每周皮下注射致癌物质N-亚硝基双（2-氧代丙基）胺（BOP），共注射5次（第一次，70mg/kg体重，其余每次20mg/kg）。接受BOP注射的仓鼠通过饲管给予0.2ml中链甘油三酯（MCT），其中一组添加0.04μg的1α-羟基维生素D3 [1α(OH)D3]，另一组不添加，持续12周。因此，分为3组：第1组；仅BOP组（n＝20），第2组；BOP＋MCT组（n＝18），第3组；BOP＋1α(OH)D3组（n＝25）。实验结束时，第3组的平均体重低于第1组和第2组（p＜0.001，Tukey-Kramer HSD检验）。在第12周结束时，所有存活的仓鼠均实施安乐死。第1组、第2组和第3组的肝肿瘤发生率分别为80%、72%和32%。第3组的肿瘤发生率显著低于第1组和第2组（p＜0.05，χ2检验）。所有肿瘤均为胆管癌。这些结果表明，补充1α(OH)D3可抑制BOP诱导的胆管癌发生。
