Birt D F, Pour P M
J Natl Cancer Inst. 1985 May;74(5):1121-7.
Syrian golden hamsters were fed four diets in experiments designed to evaluate the effects of the interaction of dietary fat and protein on carcinogenesis induced by N-nitrosobis(2-oxopropyl)amine [(BOP) CAS: 60599-38-4]. The diets consisted of two levels of dietary fat [4.5 g (low fat, LF) or 18 g (high fat, HF) of corn oil/385 kilocalories (kcal)]. These levels were fed with each of two levels of dietary protein [9 g low-protein (LP) and 36 g high-protein (HP) casein/385 kcal]. The four diets were fed to two separate groups of hamsters at two different periods in their life-span. For testing of the effects of diet on tumor initiation, one group received the diets from 3 to 7 weeks of age. At 8 weeks, they were given injections sc of 10 mg BOP/kg body weight and placed on a control diet [9 g corn oil (medium fat) and 18 g casein (medium protein)/385 kcal]. The other group received control diet until 8 weeks of age, at which time they were given injections of BOP and placed on the four diets. This group was designed to test the effects of the diets on tumor development. BOP-induced lesions in the lungs, liver, common bile duct, gallbladder, and kidneys are described; results in the pancreas were reported separately. In hamsters fed the four diets after BOP treatment, the LF-LP groups had the fewest tumors, the LF-HP-fed and HF-LP-fed groups had intermediate yields of tumors, and the hamsters given HF-HP diet exhibited the largest numbers of neoplasms. Several specific tumor types showed a similar pattern. For example, the pulmonary adenoma incidence, which was low in the non-BOP-treated hamsters, was higher in the HF-HP group than in those fed LF-HP diet after BOP, but it was not influenced by fat at the LP level. In addition, renal adenomas were observed at a low incidence in non-BOP-treated hamsters and in hamsters fed LF-LP levels before or after BOP treatment (0.5% incidence) but were present at an 8% incidence in all other BOP-treated groups. The incidence of biliary cystic adenomas was highest in male hamsters that received HF diets, irrespective of BOP treatment, and BOP treatment resulted in increased yields of this lesion in females only in groups given HF-LP diet.(ABSTRACT TRUNCATED AT 400 WORDS)
在旨在评估膳食脂肪和蛋白质的相互作用对N-亚硝基双（2-氧代丙基）胺[（BOP），化学物质登录号：60599-38-4]诱导的致癌作用影响的实验中，给叙利亚金黄地鼠喂食四种不同的饮食。这些饮食包含两种水平的膳食脂肪[4.5克（低脂，LF）或18克（高脂，HF）玉米油/385千卡（kcal）]。这两种脂肪水平分别与两种水平的膳食蛋白质[9克低蛋白（LP）和36克高蛋白（HP）酪蛋白/385千卡]搭配。在叙利亚金黄地鼠寿命的两个不同时期，将这四种饮食分别喂给两组不同的地鼠。为了测试饮食对肿瘤起始的影响，一组地鼠在3至7周龄时接受这些饮食。8周时，给它们皮下注射10毫克BOP/千克体重，并改为喂食对照饮食[9克玉米油（中等脂肪）和18克酪蛋白（中等蛋白质）/385千卡]。另一组地鼠在8周龄前接受对照饮食，此时给它们注射BOP，然后改为喂食这四种饮食。该组用于测试饮食对肿瘤发展的影响。描述了BOP诱导的肺部、肝脏、胆总管、胆囊和肾脏的病变；胰腺的结果单独报告。在BOP处理后喂食四种饮食的地鼠中，LF-LP组的肿瘤最少，LF-HP组和HF-LP组的肿瘤产量中等，而喂食HF-HP饮食的地鼠肿瘤数量最多。几种特定的肿瘤类型也呈现出类似的模式。例如，在未接受BOP处理的地鼠中肺腺瘤发病率较低，在BOP处理后，HF-HP组的肺腺瘤发病率高于喂食LF-HP饮食的地鼠，但在LP水平时不受脂肪影响。此外，在未接受BOP处理的地鼠以及在BOP处理前后喂食LF-LP水平饮食的地鼠中，肾腺瘤的发病率较低（0.5%），但在所有其他接受BOP处理的组中发病率为8%。无论是否接受BOP处理，接受HF饮食的雄性地鼠胆囊性腺瘤的发病率最高，并且仅在喂食HF-LP饮食的组中，BOP处理会导致雌性地鼠中这种病变的产量增加。（摘要截断于400字）
